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Development of Amylomaize Hybrids using GEM Germplasm

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1 Development of Amylomaize Hybrids using GEM Germplasm
Mark Campbell, Andrew Green, Joseph Bell (Truman State University) Jay-lin Jane, Hongxin Jiang (Iowa State University) Yusheng WU (South Dakota State University) Chicago ASTA meeting– December 5th 2007

2

3 Applications of high-amylose starch…..
Gum candies Adhesives Biodegradable plastics Food coatings

4 Resistant Starch- a nutraceutical
Normal starch Resistant starch Cold potatoes Green bananas Novel industrial synthetic sources High amylose corn

5 Resistant Starch- a nutraceutical
Normal starch Resistant starch Cold potatoes Green bananas Novel industrial synthetic sources High amylose corn

6 Resistant starch feeds bifidobacterium in large intestine
Butyrate is good for us!!!!! -decreases colon cancer cell formation -decreases cholesterol -lowers blood pressure -weight control

7 ae – amylose extender

8 How to Develop Amylomaize VII Class Hybrids from Scratch:
First step – Find ‘modifier genes’

9 Screened many ae-type F2 in the lab
Plant introductions GEM breeding crosses Visually selected ae types kernels Screened many ae-type F2 in the lab

10 GUAT209:S13//OH43ae/H99ae GEMS-0067 is now freely available!!

11 Next……Hybrid Development
From GEMS-0067 both SS and NSS parents must be made

12 SS inbreds (S1) NSS S1 inbreds (S1)

13 SS inbreds CHIS775:S1911b B-B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae CUBA164:S B-B-sib////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae ( _SE32_S17_F2S4_9148-Blk22/00-sib///GUAT209:S13//OH43ae/H99ae1-2-1) - 1 CUBA164:S1511b B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae DKB844:S B-B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae UR11003:S B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae CUBA164:S B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae UR11003:S B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae (CHIS740:S1411a B-B /// GUAT209:S13//OH43ae/H99ae (CUBA164:S1511b B///GUAT209:S13//OH43ae/H99ae ) - 1 (UR10001:S ///GUAT209:S13//OH43ae/H99ae ) - 1 (FS8A(S):S B///GUAT209:S13//OH43ae/H99ae ) - 1 (CUBA164:S B///GUAT209:S13//OH43ae/H99ae ) - 1 (AR16026:S B///GUAT209:S13//OH43ae/H99ae ) - 1 ( _DK888_S11_F2S4_9205-Blk24/00-sib///GUAT209:S13//OH43ae/H99ae ) - 1

14 NSS inbreds DKXL370:N11a B-B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae DXKL370:N11a B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae DKXL370:N11a B-B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae CH05015:N B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae AR03056:N B-B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae DK212T:N11a B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae BARBGP2:N08a B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae FS8B(T):N11a-87-1-B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae CH05015:N B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae UR13085:N B-B-B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae DREP150:N2011d B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae FS8B(T):N11a B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae (DKXL370:N11a B-B-B /// GUAT209:S13//OH43ae/H99ae FS8B(T):N11a B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae (CH05015:N B-B///GUAT209:S13//OH43ae/H99ae ) - 1 (DKXL370:N11a B///GUAT209:S13//OH43ae/H99ae ) - 1 (UR13085:N B///GUAT209:S13//OH43ae/H99ae ) - 1 (CH05015:N B-B///GUAT209:S13//OH43ae/H99ae ) - 1 (SCR01:N B-B///GUAT209:S13//OH43ae/H99ae ) - 1

15 Yield trial – 2007 83 GEM (SS) x GEM(NSS) entries
2 proprietary hybrids obtained from a company whose name must not be spoken

16 Pedigree Average Performance over MO and IA locations Yield
Entry Pedigree Average Performance over MO and IA locations Yield (bu/ac) MOIST TWTa 14 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 111.8 27.2 52.4 10 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 111.7 25.9 47.9 42 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 108.6 28.0 49.9 50 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 106.7 27.1 49.4 15 (DXKL370:N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 106.5 25.3 50.9 3 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 106.3 27.3 48.0 23 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 103.5 26.4 21 (BARBGP2:N08a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 102.3 24.9 49.7 43 (DXKL370:N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 101.9 26.8 47.0 8 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 101.8 25.7 61 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 101.2 26.9 52.2 11 (DK212T:N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 100.2 29.2 46.5 51 (FS8B(T):N11a-87-1-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 100.1 48.1 40 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 99.6 28.6 50.4 52 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 99.5 26.3 50.8 1 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 99.0 28.8 48.9 80 (( _SE32_S17_F2S4_9148-Blk22/00-sib///GM67//O/Hae1-2-1) - 1 )x((CH05015:N B-B///GM67//O/Hae 1-2-1) - 1) 98.3 25.6 49.6 39 (UR13085:N B-B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 98.2 25 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 98.1 49.2 12 97.6 27.8 20 (UR13085:N B-B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 96.7 48.4 77 (( _SE32_S17_F2S4_9148-Blk22/00-sib///GM67//O/Hae1-2-1) - 1 )x((SCR01:N B-B///GM67//O/Hae ) - 1) 96.3 9 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 95.6 25.0 48.8 62 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 95.3 27.7 46 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 94.6 29 (FS8B(T):N11a-87-1-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 94.0 28.3 27 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 93.5 29.3 50.7 28 (DXKL370:N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 93.4 51.5 57 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 92.8 47.4 6 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 92.4 26.6 48.3 24 (DK212T:N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 47.2 47 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 91.4 29.0 48.5 59 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 91.3 49.8 60 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 91.2 47.7 63 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 27.5 50.1 48 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 89.6 48.6 58 89.3 27.0 46.6 70 (DREP150:N2011d B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 27.6 45 (UR13085:N B-B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 89.1 29.1 49 (DK212T:N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 88.7 27.9 47.6 53 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 88.4 28.2 49.0 16 (FS8B(T):N11a-87-1-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 88.0 37 (DREP150:N2011d B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 87.9 36 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 87.7 41 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 87.4 24.1 51.0 72 Check 1 87.1 84 ((CUBA164:S B///GM67//O/Hae1-2-1) - 1 )x((CH05015:N B-B///GM67//O/Hae ) - 1) 86.9 24.7 2 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 86.8 26.5 67 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 86.2 30 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 86.1 52.8 34 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 85.4 68 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 25.8 65 (BARBGP2:N08a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 85.3 26.2 35 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 85.2 28.1 48.2 78 ((CHIS740:S1411a B-B /// GM67//O/Hae )x((DKXL370:N11a B-B-B /// GM67//O/Hae ) 84.9 22 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 84.3 19 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 28.9 71 73 ((CUBA164:S1511b B///GM67//O/Hae ) - 1 )x((CH05015:N B-B///GM67//O/Hae ) - 1) 83.8 24.3 64 (UR13085:N B-B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 83.5 25.4 49.3 85 Check 2 32 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 83.3 69 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 82.9 23.7 50.0 26 (DREP150:N2011d B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 82.8 51.2 54 82.7 4 (DXKL370:N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 82.5 18 82.4 44 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 82.2 26.7 17 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 80.6 25.2 83 (( _SE32_S17_F2S4_9148-Blk22/00-sib///GM67//O/Hae1-2-1) - 1 )x((DKXL370:N11a B///GM67//O/Hae ) - 1) 80.5 47.8 31 (UR13085:N B-B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 80.1 48.7 82 ((FS8A(S):S B///GM67//O/Hae ) - 1 )x((UR13085:N B///GM67//O/Hae ) - 1) 79.7 56 (BARBGP2:N08a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 78.2 81 ((CH05015:N B-B///GM67//O/Hae ) - 1 )x((CUBA164:S B///GM67//O/Hae ) - 1) 78.0 25.1 38 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 77.9 49.5 79 ((CH05015:N B-B///GM67//O/Hae ) - 1 )x((UR10001:S ///GM67//O/Hae ) - 1) 26.1 47.1 74 ((AR16026:S B///GM67//O/Hae ) - 1 )x((CH05015:N B-B///GM67//O/Hae ) - 1) 77.5 55 (UR13085:N B-B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 76.9 13 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 75.4 66 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 74.3 24.6 33 (DK212T:N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 46.9 5 (FS8B(T):N11a-87-1-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 71.2 47.5 7 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 70.0 45.7 76 (( _DK888_S11_F2S4_9205-Blk24/00-sib///GM67//O/Hae ) - 1 )x((CH05015:N B-B///GM67//O/Hae ) - 1) 68.9 26.0 75 ((CUBA164:S B///GM67//O/Hae ) - 1 )x((SCR01:N B-B///GM67//O/Hae ) - 1) 52.9 mean of entries across location and replication 88.9  LSD 27.8  3.57   3.3

17 LSD mean of entries across location and replication 91.3 91.2 89.6
59 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 91.3 27.3 49.8 60 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 91.2 27.7 47.7 63 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 27.5 50.1 48 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 89.6 26.9 48.6 58 89.3 27.0 46.6 70 (DREP150:N2011d B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 27.6 49.7 45 (UR13085:N B-B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 89.1 29.1 47.2 49 (DK212T:N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 88.7 27.9 47.6 53 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 88.4 28.2 49.0 16 (FS8B(T):N11a-87-1-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 88.0 26.8 48.0 37 (DREP150:N2011d B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 87.9 28.8 48.3 36 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 87.7 48.9 41 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 87.4 24.1 51.0 72 Check 1 87.1 25.0 49.2 84 ((CUBA164:S B///GM67//O/Hae1-2-1) - 1 )x((CH05015:N B-B///GM67//O/Hae ) - 1) 86.9 24.7 2 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 86.8 26.5 67 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 86.2 24.9 48.8 30 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 86.1 52.8 34 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 85.4 28.6 50.4 68 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 25.8 65 (BARBGP2:N08a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 85.3 26.2 50.7 35 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 85.2 28.1 48.2 78 ((CHIS740:S1411a B-B /// GM67//O/Hae )x((DKXL370:N11a B-B-B /// GM67//O/Hae ) 84.9 51.5 22 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 84.3 19 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 28.9 71 48.4 73 ((CUBA164:S1511b B///GM67//O/Hae ) - 1 )x((CH05015:N B-B///GM67//O/Hae ) - 1) 83.8 24.3 49.6 64 (UR13085:N B-B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 83.5 25.4 49.3 85 Check 2 25.6 49.9 32 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 83.3 69 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 82.9 23.7 50.0 26 (DREP150:N2011d B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 82.8 28.3 51.2 54 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 82.7 4 (DXKL370:N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 82.5 25.7 18 82.4 27.2 44 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b B-B-B////AR16035:S B-B///GM67//O/Hae) 82.2 26.7 17 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B-sib////AR16035:S B-B///GM67//O/Hae) 80.6 25.2 83 (( _SE32_S17_F2S4_9148-Blk22/00-sib///GM67//O/Hae1-2-1) - 1 )x((DKXL370:N11a B///GM67//O/Hae ) - 1) 80.5 47.8 31 (UR13085:N B-B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 80.1 48.7 82 ((FS8A(S):S B///GM67//O/Hae ) - 1 )x((UR13085:N B///GM67//O/Hae ) - 1) 79.7 56 (BARBGP2:N08a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 78.2 81 ((CH05015:N B-B///GM67//O/Hae ) - 1 )x((CUBA164:S B///GM67//O/Hae ) - 1) 78.0 25.1 38 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 77.9 49.5 79 ((CH05015:N B-B///GM67//O/Hae ) - 1 )x((UR10001:S ///GM67//O/Hae ) - 1) 26.1 47.1 74 ((AR16026:S B///GM67//O/Hae ) - 1 )x((CH05015:N B-B///GM67//O/Hae ) - 1) 77.5 55 (UR13085:N B-B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 76.9 27.8 48.1 13 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 75.4 26.3 66 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 74.3 24.6 33 (DK212T:N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 46.9 5 (FS8B(T):N11a-87-1-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 71.2 47.5 7 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 70.0 45.7 76 (( _DK888_S11_F2S4_9205-Blk24/00-sib///GM67//O/Hae ) - 1 )x((CH05015:N B-B///GM67//O/Hae ) - 1) 68.9 26.0 75 ((CUBA164:S B///GM67//O/Hae ) - 1 )x((SCR01:N B-B///GM67//O/Hae ) - 1) 52.9 mean of entries across location and replication 88.9  LSD 27.8  3.57   3.3

18 Yield (ave.) Yield (Max) Moist (ave) 68.3 95.4 26.2 109.5 148.9 27.3
83 (( _SE32_S17_F2S4_9148-Blk22/00-sib///GM67//O/Hae1-2-1) - 1 )x((DKXL370:N11a B///GM67//O/Hae ) - 1) 80.5 25.2 47.8 31 (UR13085:N B-B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 80.1 28.3 48.7 82 ((FS8A(S):S B///GM67//O/Hae ) - 1 )x((UR13085:N B///GM67//O/Hae ) - 1) 79.7 26.2 48.9 56 (BARBGP2:N08a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 78.2 26.9 49.6 81 ((CH05015:N B-B///GM67//O/Hae ) - 1 )x((CUBA164:S B///GM67//O/Hae ) - 1) 78.0 25.1 48.2 38 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 77.9 49.5 79 ((CH05015:N B-B///GM67//O/Hae ) - 1 )x((UR10001:S ///GM67//O/Hae ) - 1) 26.1 47.1 74 ((AR16026:S B///GM67//O/Hae ) - 1 )x((CH05015:N B-B///GM67//O/Hae ) - 1) 77.5 24.7 47.2 55 (UR13085:N B-B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(DKB844:S B-B-B////AR16035:S B-B///GM67//O/Hae) 76.9 27.8 48.1 13 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 75.4 26.3 66 (CH05015:N B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(UR11003:S B-B////AR16035:S B-B///GM67//O/Hae) 74.3 24.6 50.0 33 (DK212T:N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S B-B////AR16035:S B-B///GM67//O/Hae) 28.1 46.9 5 (FS8B(T):N11a-87-1-B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 71.2 25.4 47.5 7 (FS8B(T):N11a B-B////DKXL370:N11a B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b B-B////AR16035:S B-B///GM67//O/Hae) 70.0 45.7 76 (( _DK888_S11_F2S4_9205-Blk24/00-sib///GM67//O/Hae ) - 1 )x((CH05015:N B-B///GM67//O/Hae ) - 1) 68.9 26.0 75 ((CUBA164:S B///GM67//O/Hae ) - 1 )x((SCR01:N B-B///GM67//O/Hae ) - 1) 52.9 mean of entries across location and replication 88.9 26.8 49.0  LSD 27.8  3.57   3.3 Yield (ave.) Yield (Max) Moist (ave) Missouri 68.3 95.4 26.2 Iowa 109.5 148.9 27.3

19 Five entries were found on IA and MO list of top 15 entries by yield.
14 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GEMS0067//O/H ae x CUBA164:S B-B-sib////AR16035:S B-B///GEMS0067//O/H ae) 138.7 85.0 42 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GEMS0067//O/H ae x CHIS775:S1911b B-B-B////AR16035:S B-B///GEMS0067//O/H ae) 137.6 79.6 50 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GEMS0067//O/H ae x CHIS775:S1911b B-B-B////AR16035:S B-B///GEMS0067//O/H ae) 134.8 78.6 10 (AR03056:N B-B-B////DKXL370:N11a B-B-SIB///GEMS0067//O/H ae x CUBA164:S1511b B-B////AR16035:S B-B///GEMS0067//O/H ae) 123.8 95.4 61 (DKXL370:N11a B-B-B////DKXL370:N11a B-B-SIB///GEMS0067//O/H ae x DKB844:S B-B-B////AR16035:S B-B///GEMS0067//O/H ae) 125.2 77.3

20 Averaged performance of SS lines by yield
SS code SS inbred n Yield (bu/ac) MOIST TWT 10 CHIS775:S1911b B-B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 11 94.5 27.2 49.0 12 CUBA164:S B-B-sib////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 13 93.4 27.0 49.2 1 ( _SE32_S17_F2S4_9148-Blk22/00-sib///GUAT209:S13//OH43ae/H99ae1-2-1) - 1 2 91.7 25.3 49.4 CUBA164:S1511b B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 91.6 26.6 47.8 14 DKB844:S B-B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 90.1 27.1 16 UR11003:S B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 88.1 CUBA164:S B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 86.5 28.1 49.6 15 UR11003:S B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 85.8 26.1 49.7 4 (CHIS740:S1411a B-B /// GUAT209:S13//OH43ae/H99ae 84.9 27.3 51.5 5 (CUBA164:S1511b B///GUAT209:S13//OH43ae/H99ae ) - 1 83.8 24.3 9 (UR10001:S ///GUAT209:S13//OH43ae/H99ae ) - 1 80.7 25.9 48.5 8 (FS8A(S):S B///GUAT209:S13//OH43ae/H99ae ) - 1 3 80.4 26.0 48.6 7 (CUBA164:S B///GUAT209:S13//OH43ae/H99ae ) - 1 6 (CUBA164:S B///GUAT209:S13//OH43ae/H99ae ) - 1 78.0 25.1 48.2 (AR16026:S B///GUAT209:S13//OH43ae/H99ae ) - 1 77.5 24.7 47.2 ( _DK888_S11_F2S4_9205-Blk24/00-sib///GUAT209:S13//OH43ae/H99ae ) - 68.9 48.7

21 Averaged performance of SS lines by yield
SS code SS inbred n Yield (bu/ac) MOIST TWT 10 CHIS775:S1911b B-B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 11 94.5 27.2 49.0 12 CUBA164:S B-B-sib////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 13 93.4 27.0 49.2 1 ( _SE32_S17_F2S4_9148-Blk22/00-sib///GUAT209:S13//OH43ae/H99ae1-2-1) - 1 2 91.7 25.3 49.4 CUBA164:S1511b B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 91.6 26.6 47.8 14 DKB844:S B-B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 90.1 27.1 16 UR11003:S B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 88.1 CUBA164:S B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 86.5 28.1 49.6 15 UR11003:S B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 85.8 26.1 49.7 4 (CHIS740:S1411a B-B /// GUAT209:S13//OH43ae/H99ae 84.9 27.3 51.5 5 (CUBA164:S1511b B///GUAT209:S13//OH43ae/H99ae ) - 1 83.8 24.3 9 (UR10001:S ///GUAT209:S13//OH43ae/H99ae ) - 1 80.7 25.9 48.5 8 (FS8A(S):S B///GUAT209:S13//OH43ae/H99ae ) - 1 3 80.4 26.0 48.6 7 (CUBA164:S B///GUAT209:S13//OH43ae/H99ae ) - 1 6 (CUBA164:S B///GUAT209:S13//OH43ae/H99ae ) - 1 78.0 25.1 48.2 (AR16026:S B///GUAT209:S13//OH43ae/H99ae ) - 1 77.5 24.7 47.2 ( _DK888_S11_F2S4_9205-Blk24/00-sib///GUAT209:S13//OH43ae/H99ae ) - 68.9 48.7

22 SS inbred n (bu/ac) MOIST Yield TWT
SS code SS inbred n (bu/ac) Yield MOIST TWT 10 CHIS775:S1911b B-B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 11 94.5 27.2 49.0 12 CUBA164:S B-B-sib////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 13 93.4 27.0 49.2 1 ( _SE32_S17_F2S4_9148-Blk22/00-sib///GUAT209:S13//OH43ae/H99ae1-2-1) - 1 2 91.7 25.3 49.4 CUBA164:S1511b B-B////AR16035:S B-B///GUAT209:S13//OH43ae/H99ae 91.6 26.6 47.8

23 Averaged performance of SS lines by yield
NS code NS line n Yield (bu/ac) MOIST TWT 29 DKXL370:N11a B-B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 2 107.4 27.7 48.9 31 DXKL370:N11a B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 4 96.1 26.2 49.5 28 DKXL370:N11a B-B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 6 94.4 26.6 50.7 26 CH05015:N B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 5 94.2 26.9 48.7 23 AR03056:N B-B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 17 92.6 27.3 27 DK212T:N11a B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 88.9 28.4 47.0 24 BARBGP2:N08a B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 3 88.6 26.0 50.0 34 FS8B(T):N11a-87-1-B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 88.3 48.2 25 CH05015:N B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 87.7 49.3 35 UR13085:N B-B-B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 87.4 27.5 48.5 30 DREP150:N2011d B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 86.7 28.2 49.7 32 FS8B(T):N11a B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 85.3 25.2 48.6 19 (DKXL370:N11a B-B-B /// GUAT209:S13//OH43ae/H99ae 1 84.9 51.5 33 FS8B(T):N11a B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 9 84.0 49.6 18 (CH05015:N B-B///GUAT209:S13//OH43ae/H99ae ) - 1 83.2 25.1 49.1 20 (DKXL370:N11a B///GUAT209:S13//OH43ae/H99ae ) - 1 80.5 47.8 22 (UR13085:N B///GUAT209:S13//OH43ae/H99ae ) - 1 79.7 (CH05015:N B-B///GUAT209:S13//OH43ae/H99ae ) - 1 77.9 25.6 47.7 21 (SCR01:N B-B///GUAT209:S13//OH43ae/H99ae ) - 1 74.6

24 Averaged performance of SS lines by yield
NS code NS line n Yield (bu/ac) MOIST TWT 29 DKXL370:N11a B-B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 2 107.4 27.7 48.9 31 DXKL370:N11a B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 4 96.1 26.2 49.5 28 DKXL370:N11a B-B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 6 94.4 26.6 50.7 26 CH05015:N B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 5 94.2 26.9 48.7 23 AR03056:N B-B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 17 92.6 27.3 27 DK212T:N11a B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 88.9 28.4 47.0 24 BARBGP2:N08a B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 3 88.6 26.0 50.0 34 FS8B(T):N11a-87-1-B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 88.3 48.2 25 CH05015:N B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 87.7 49.3 35 UR13085:N B-B-B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 87.4 27.5 48.5 30 DREP150:N2011d B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 86.7 28.2 49.7 32 FS8B(T):N11a B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 85.3 25.2 48.6 19 (DKXL370:N11a B-B-B /// GUAT209:S13//OH43ae/H99ae 1 84.9 51.5 33 FS8B(T):N11a B-B////DKSL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 9 84.0 49.6 18 (CH05015:N B-B///GUAT209:S13//OH43ae/H99ae ) - 1 83.2 25.1 49.1 20 (DKXL370:N11a B///GUAT209:S13//OH43ae/H99ae ) - 1 80.5 47.8 22 (UR13085:N B///GUAT209:S13//OH43ae/H99ae ) - 1 79.7 (CH05015:N B-B///GUAT209:S13//OH43ae/H99ae ) - 1 77.9 25.6 47.7 21 (SCR01:N B-B///GUAT209:S13//OH43ae/H99ae ) - 1 74.6

25 NS code NS - lines n Yield (bu/ac) MOIST 29 DKXL370:N11a B-B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 2 107.4 27.7 31 DXKL370:N11a B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 4 96.1 26.2 28 DKXL370:N11a B-B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 6 94.4 26.6 26 CH05015:N B-B////DKXL370:N11A B-B///GUAT209:S13//OH43ae/H99ae 5 94.2 26.9

26 Studies on the inheritance and gene action of high amylose modifier genes
High amylose modifiers often treated multiple genes that influence a quantitative trait. V. Fergason, 1994 In: Specialty Corns’

27 Generation Means Analysis
A triploid model proposed by Bogyo et al (1988) is being employed GEMS-0067, H99ae and the F1 were selfed and intermated to produce 9 distinct generations.

28 Amylose based on colorimetric method
Source DF MS F Population 9 49.14** Location 1 448.90 21.61** Year 131.10 6.35** Pop × Loc 49.10 2.38** Pop × Year 4 59.80 2.90** Loc × Year 2.24 0.11 Pop × Loc × Year 30.60 1.48 Error 1240 20.63 **: significant at 1% probability level.

29 Amylose based on gel permeation chromatography at ISU
GEMS-0067 Amylose based on gel permeation chromatography at ISU amylose amylopectin H99 ae

30

31 Normal starch Corn with ae allele (oC)

32

33

34

35 QTL analysis of high amylose modifier genes

36

37 amylose-extender (ae) locus
High amylose modifier? amylose-extender (ae) locus

38

39

40

41

42 Obtained from Mark Guiltinan, Penn State using the Trait Utility System for Corn (TUSC) developed by Pioneer Hi-Bred International, Inc. sbe1a-Mu (W64A-BC4); ae self sbe2a-Mu (W64A-BC4) self ae

43

44 Distribution of amylose values among F2 ears in the GEMS-0067 x H99ae mapping populations
Appears to be more than one gene involved

45 Question?

46

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