1. Administration of βHCG leads to dose-dependent changes of gene expression signature of endometriotic stromal cells 
Gernot Hudelist, Ambros Huber, Michael Auer, Martin Knöfler, Leila Saleh, Christian Hrachowitz, Johannes C. Huber, Ernst Kubista, Mahmood Manavi, Martin Bilban, Christian F. Singer 
Reproductive BioMedicine Online 
Volume 20, Issue 5, Pages (May 2010)
DOI: /j.rbmo
Copyright © Terms and Conditions

2. Figure 1
Fluorescence immunohistochemistry of monocultures of stromal fibroblasts using (A) CD45 antibody, (C) CD56 antibody and (E) vimentin antibody. (B, D and F) Corresponding DAPI nuclear staining controls.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © Terms and Conditions

3. Figure 2
(Left panel) Identification of clusters of genes with a similar change in mRNA expression in response to increasing concentrations of HCG after 12h of exposure: lane S_1a, 0U/ml; S_2a, 0.1U/ml; S_3a, 1U/ml; S_4a, 10U/ml; S_5a, 50U/ml; S_6, 100U/ml. (Right panel) Corresponding logarithmic representation of gene expression: lane 1, 0U/ml; 2, 0.1U/ml; 3, 1U/ml; 4, 10U/ml; 5, 50U/ml; 6, 100U/ml. Each line represents mRNA expression of one gene. Genes depicted in red represent down-regulated genes, while genes depicted in blue represent up-regulated genes. Some genes, such as EMP1, are represented in both clusters 1 and 3 because, in a small number of cases, the analysis software attributes expression levels that show a borderline expression pattern into two categories. Some genes appear more than once in the same cluster due to slightly different results from replicate measurements causing the software to print each of the slightly different arrays.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © Terms and Conditions

4. Figure 3
Bar graph reflecting percentage distribution (Y-axis) of cluster 1 (light grey bar), cluster 2 (dark grey bar), cluster 3 (white bar) according to their function (X-axis) in cell adhesion and communication (1), extracellular matrix remodelling (2), proapoptotic mechanisms (3), anti-inflammatory processes (4), down-stream receptor signalling (5), cell cycle control (6) and cell growth and transcriptional processes (7).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © Terms and Conditions

5. Figure 4
Real-time polymerase chain reaction for (A) PAI2, (B) DUSP6, (C) PLAU and (D) MMP1 performed on total RNA extracted from nine monocultures of stromal cells obtained from peritoneal endometriotic lesions which were incubated without (□) or with (■) 50U/ml βHCG for 12 h.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © Terms and Conditions