1. Partial knockdown of MPST markedly ameliorates hepatic steatosis in vivo.
Partial knockdown of MPST markedly ameliorates hepatic steatosis in vivo. (A–C) HFD-fed C57/BL6 mice were given 1×109 plaque-forming units of AD-GFP or adenovirus-mediated knockdown of MPST (AD-shMPST) via the tail vein every 2 weeks from the beginning of the experiment and were sacrificed after 8 weeks of HFD feeding. (A) Liver tissues were subjected to western blot analyses of MPST protein level (upper panel) or real-time qPCR for MPST mRNA level determination (lower panel). (B) Representative images of H&E and Oil Red O staining of livers infected with AD-GFP and AD-shMPST in HFD-fed mice. Original magnification ×400. (C) Liver tissues were subjected to intrahepatic FFA measurement (left panel), intrahepatic TG measurement (middle panel) or intrahepatic TC measurement (right panel). (D–F) MPST+/+ and MPST+/- mice were fed an HFD for 8 weeks. (D) Liver tissues were subjected to western blot analyses of MPST protein level (upper panel) or real-time qPCR for MPST mRNA level determination (lower panel). (E) Representative images of H&E and Oil Red O staining of liver sections in HFD-fed MPST+/+ and MPST+/- mice. Original magnification ×400. (F) Liver tissues were subjected to intrahepatic FFA measurement (left panel), intrahepatic TG measurement (middle panel) or intrahepatic TC measurement (right panel). The results are expressed as the mean ±SD of 3–5 mice per group from two independent experiments. *p<0.05, **p<0.01, ***p< FFA, free fatty acid; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HFD, high fat diet; MPST, 3-mercaptopyruvate sulfurtransferase; qPCR, quantitative PCR; TC, total cholesterol; TG, triglyceride.
Meng Li et al. Gut 2018;67:
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