1. Meiotic arrest at the midpachytene stage in a patient with complete azoospermia factor b deletion of the Y chromosome 
Jeanne Perrin, M.D., Catherine Metzler-Guillemain, M.D., Ph.D., Gilles Karsenty, M.D., Jean-Marie Grillo, M.D., Ph.D., Michael J. Mitchell, Ph.D., Marie-Roberte Guichaoua, M.D., Ph.D. 
Fertility and Sterility 
Volume 85, Issue 2, Pages 494.e5-494.e8 (February 2006)
DOI: /j.fertnstert
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
Spermatocyte nuclei stained for SCP3. Staging is by XY configuration (arrows: XY bivalent). (A, B, D) Analysis performed with a fluorescent photomicroscope. (C, E, F) Cells mounted with DAPI, analysis performed with a fluorescent microscope equipped with a charge-coupled device camera, images collected and merged with commercial software. (A) Leptotene spermatocyte showing discontinuous axial elements. (B) Normal stage II pachytene spermatocyte. (C) Normal stage III pachytene spermatocyte. (D) Abnormal zygotene/pachytene spermatocyte showing extended asynapsis. (E) Abnormal pachytene spermatocyte showing fragmented synaptonemal complexes. (F) Abnormal pachytene spermatocyte showing dotted synaptonemal complexes.
Perrin. Meiotic study in AZFb microdeletion. Fertil Steril 2006.
Fertility and Sterility  , 494.e5-494.e8DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Spermatocyte nuclei stained for SCP3 (green) and γH2AX (red). (A) Analysis performed with a fluorescent photomicroscope. (B, B′, C, D, D′) Cells mounted with DAPI, analysis performed with a fluorescent microscope equipped with a charge-coupled device camera, images collected and merged using commercial software. (A) Leptotene-stage spermatocyte showing normal patchy γH2AX staining. (B) Normal stage II pachytene spermatocyte staged by XY configuration (arrow). (B′) Same nucleus with γH2AX staining restricted to the XY body. (C) Pachytene spermatocyte with fragmented synaptonemal complexes and normal γH2AX staining. (D) Asynapsed zygotene/pachytene nucleus with SCP3 staining. (D′) Same nucleus with γH2AX “patches.”
Perrin. Meiotic study in AZFb microdeletion. Fertil Steril 2006.
Fertility and Sterility  , 494.e5-494.e8DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions