1. Successful slush nitrogen vitrification of human ovarian tissue
Riccardo Talevi, Ph.D., Vincenza Barbato, Ph.D., Ilaria Fiorentino, Ph.D., Sabrina Braun, Ph.D., Cristofaro De Stefano, M.D., Raffaele Ferraro, M.D., Sam Sudhakaran, M.Sc., Roberto Gualtieri, Ph.D. 
Fertility and Sterility 
Volume 105, Issue 6, Pages e1 (June 2016)
DOI: /j.fertnstert
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Distribution of human primordial/primary grade 1–3 follicles in hematoxilin/eosin-stained sections of fresh and vitrified ovarian tissue. CONTR = fresh control. **P<.01 vs. fresh control; ##P<.01 vs. LN.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
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3. Figure 2
Transmission electron micrographs of representative follicles in fresh (A, B) and vitrified ovarian tissue with liquid (C, D) or slush (E, F) nitrogen. Arrowhead, damaged GCs. In A, C, and E, bar = 4 μm; in B, D, and F, bar = 2 μm.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Transmission electron micrographs of GCs (A, C, E) and SCs (B, D, F) in fresh (A, B) and vitrified ovarian tissue with liquid (C, D) or slush (E, F) nitrogen. Arrowhead, damaged GC. Asterisks (*) indicate damaged SCs. In A, C, and E, bar = 2 μm; in B, D, and F, bar = 4 μm.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Representative confocal micrographs of ovarian tissue viability (red: live–dead far-red probe; blue: Hoechst 33342–stained nuclei). (A) Low magnification of three follicles with viable oocytes (asterisk [*] indicates dead GC). (B) High magnification of a follicle with a dead oocyte (arrowhead indicates the germinal vesicle). (C, D) High magnifications of follicles with live oocytes (asterisk indicates dead GC). (E, F) High magnification of LN (E) and SN (F) vitrified ovarian stroma with dead SCs in pink (overlap of the nuclear stain and live–dead far-red probe). Bar = 25 μm.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions