1. Sperm nuclear vacuoles, as assessed by motile sperm organellar morphological examination, are mostly of acrosomal origin 
O Kacem, C Sifer, V Barraud-Lange, B Ducot, D De Ziegler, C Poirot, JP Wolf 
Reproductive BioMedicine Online 
Volume 20, Issue 1, Pages (January 2010)
DOI: /j.rbmo
Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

2. Figure 1
Acrosome detection on normal spermatozoa, according to sperm organellar morphological examination. Spermatozoa were immobilized and dried on a smear. The smear was then analysed by Normaski interferential contrast (A and B) and for the acrosomal status using the Pisum sativum agglutinin–fluorescein isothiocyanate (PSA-FITC) detection technique and immunofluorescence (A′ and B′). Merge (A″ and B″). Note the absence of fluorescence after PSA-FITC staining (×6600).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2
Acrosome detection on spermatozoa with nuclear vacuoles, according to sperm organellar morphological examination. Spermatozoa with nuclear vacuoles (see Figure 1) (×6600) (A) spermatozoon with incomplete acrosome reaction, (B) acrosome intact. Immunofluorescence (A′ and B′). Merge (A″ and B″). Highlighted green spots can be seen in the location of the vacuoles (arrows). These spermatozoa had a fluorescent zone on all the anterior head suggesting an intact acrosome or incomplete acrosome reaction (×6600).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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4. Figure 3
Live motile spermatozoa analysed by motile sperm organellar morphological examination at consecutive time points. The same spermatozoon with a nuclear vacuole is shown on two consecutive pictures. Panels (A) and (B) show a live motile spermatozoon with protruding blebs (arrows) in the acrosomal region. It could be interpreted as an ongoing acrosome reaction. A different spermatozoon in shown panel (C) exhibits an embossed image. However, the same image looks like a defect of material on the same spermatozoon in (D) (arrows) (×6600).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions