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Novel use of the ovarian follicular pool to postpone menopause and delay osteoporosis
Claus Yding Andersen, Stine Gry Kristensen Reproductive BioMedicine Online Volume 31, Issue 2, Pages (August 2015) DOI: /j.rbmo Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions
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Figure 1 (A) Postponing menopause by cryopreservation of ovarian tissue. (B) Frozen-thawed human ovarian cortex from a 26-year-old-woman stained with Neutral Red in situ. All the small red dots represent viable human follicles within the cortical tissue. (C) Isolated human pre-antral follicles. An enzymatic-mix of Liberase TM, Collagenase IV and DNAse I combined with the Neutral Red stain was used to digest the ovarian tissue (Kristensen et al., 2011). (D) Isolated human follicles encapsulated in a 0.5% alginate hydrogel bead (volume = 3 µl). Reproductive BioMedicine Online , DOI: ( /j.rbmo ) Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions
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