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Published byClaire Bibeau Modified over 6 years ago
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The Applied Biosystems™ NGM Detect™ PCR Amplification Kit – As promising as promised?
S. Burch, A. Sulzer, P. Voegeli, N.V. Morf, M. Gysi, A. Kratzer Forensic Science International: Genetics Supplement Series Volume 6, Pages e504-e506 (December 2017) DOI: /j.fsigss Copyright © 2017 Elsevier B.V. Terms and Conditions
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Fig. 1 Percentage of alleles with a peak height ratio (PHR) lower than 0.6. A PHR <0.6 means that the smaller allele in a heterozygous locus is less than 60% of the height of the higher one. More alleles with PHR <0.6 means a higher allelic imbalance. Forensic Science International: Genetics Supplement Series 2017 6, e504-e506DOI: ( /j.fsigss ) Copyright © 2017 Elsevier B.V. Terms and Conditions
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Fig. 2 Number of loci amplified after DNase digestion for 30, 60 and 90s, respectively. For PP Fusion6C and GlobalFiler™ only the loci present in NGM SElect™ are shown. Dropouts start after 30s, after 90s NGM SElect™ and NGM Detect™ are still able to produce a result in at least 6 loci. Forensic Science International: Genetics Supplement Series 2017 6, e504-e506DOI: ( /j.fsigss ) Copyright © 2017 Elsevier B.V. Terms and Conditions
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Fig. 3 Details of the electropherograms of the sample with a discordance in TH01, analyzed with a) NGM Detect™, b) NGM SElect™ and c) PP ESI 17. Forensic Science International: Genetics Supplement Series 2017 6, e504-e506DOI: ( /j.fsigss ) Copyright © 2017 Elsevier B.V. Terms and Conditions
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