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Mitogenic effect of gastrin and expression of gastrin receptors in duct-like cells of rat pancreas
Ilse Rooman, Jessy Lardon, Daisy Flamez, Frans Schuit, Luc Bouwens Gastroenterology Volume 121, Issue 4, Pages (October 2001) DOI: /gast Copyright © 2001 American Gastroenterological Association Terms and Conditions
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Fig. 1 Fold increase in BrdU-labeling index of monolayers of transdifferentiated cells compared with control values after exposure to cerulein (Cae) at 10 and 100 nmol/L and to tetragastrin (TG), pentagastrin (PG), gastrin I (gasI), and sulphated gastrin II (gasII) at 0.1, 1, and 10 nmol/L. *P < 0.05. Gastroenterology , DOI: ( /gast ) Copyright © 2001 American Gastroenterological Association Terms and Conditions
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Fig. 2 Western blot of total extract from normal adult rat pancreas (Pan) and from isolated acinar cell preparations after 1, 2, 3, and 4 days of culture with a CCK-B receptor–specific antiserum. Gastroenterology , DOI: ( /gast ) Copyright © 2001 American Gastroenterological Association Terms and Conditions
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Fig. 3 (A) Immunocytochemistry for CCK-B receptor on monolayers of duct-like cells, and paraffin sections (C) of the pancreatic tail 5 days after duct ligation, (E) of fetal pancreas E17, and (F) of normal pancreas. Immunoreactivity was completely abolished after preabsorption of the antibody with the corresponding antigen in (B) cultures of duct-like cells and (D) in vivo in the ductal complexes. Gastroenterology , DOI: ( /gast ) Copyright © 2001 American Gastroenterological Association Terms and Conditions
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Fig. 4 RT-PCR analysis for CCK-B receptor mRNA and actin mRNA in RNA extracts from (lane A) adult rat pancreatic unligated head, (lane B) the ligated tail part, and (lane C) purified rat endocrine non-β cells. Gastroenterology , DOI: ( /gast ) Copyright © 2001 American Gastroenterological Association Terms and Conditions
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Fig. 5 Immunocytochemical staining for (A,C,E) amylase and (B,D,F) the duct cell marker CK20. (A and B) Starting preparations and (C–F) samples of cells (C and D) after 4 days in control cultures and (E and F) after 4 days in culture conditions with L-365,260 (100 nmol/L); bar = 30 μm. Gastroenterology , DOI: ( /gast ) Copyright © 2001 American Gastroenterological Association Terms and Conditions
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