1. Lab # 3 Aseptic Transfer Techniques

2. Aseptic Transfer Techniques
1. Protect yourself
2. Protect the environment
3. Maintain pure cultures

3. Aseptic Transfer Techniques
Aseptic – absence of organisms that may cause contamination or disease
Sterile – absence of all life

4. Aseptic Transfer Techniques
1. Sterile Pipetting
2. Aseptic Transfer with Inoculating Needle and Inoculating Loop
3. Alcohol Flaming Technique

5. TSB - Trypticase Soy Broth TSA Slant - Trypticase Soy Agar Slant
Turbid – cloudy (10 million bacterial cells per ml)
Sterilize Inoculating Needle and Loop
Always Handle to Tip - never tip to Handle
Prevents dangerous aerosols