1. O-linked N-acetylglucosamine (O-GlcNAc) protein modification is increased in the cartilage of patients with knee osteoarthritis 
L. Tardio, J. Andrés-Bergós, N.E. Zachara, A. Larrañaga-Vera, C. Rodriguez-Villar, G. Herrero-Beaumont, R. Largo 
Osteoarthritis and Cartilage 
Volume 22, Issue 2, Pages (February 2014)
DOI: /j.joca
Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

2. Fig. 1
O-GlcNAcylated protein levels and OGT and OGA presence in human healthy (white dots) and OA (black dots) knee cartilage. (A) Representative western blot and densitometric analysis for O-GlcNAcylated protein measurement using RL2 antibody. (B) Representative western blot and densitometric analysis for the three OGT isoforms, nucleo-cytoplasmic (ncOGT), mitochondrial (mOGT) and small (sOGT); and the two OGA isoforms, long (OGA-L) and short (OGA-S). EZ blue staining was used as protein loading controls. Results are expressed as fold induction for each patient, and the mean ± 95% C.I. n = 10 healthy and n = 12 OA subjects; P values calculated by Mann–Whitney test.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

3. Fig. 2
(A) O-GlcNAcylated protein levels and (B) OGT and OGA presence in HOC stimulated with IL-1α for different periods up to 24 h. Representative western blot and densitometric analysis for O-GlcNAcylated protein detection using RL2 antibody the three OGT isoforms, nucleo-cytoplasmic (ncOGT), mitochondrial (mOGT) and small (sOGT), and the two OGA isoforms, long (OGA-L) and short (OGA-S). Results are shown as fold change vs 0 h ± 95% C.I. of four independent experiments. P values calculated by Mann–Whitney test vs Basal.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions