1. Sohlh2 inhibits the apoptosis of mouse primordial follicle oocytes via C- kit/PI3K/Akt/Foxo3a signalling pathway 
Xiaoli Zhang, Haiyu Zhang, Qing Gao, Shufang Ji, Lujun Bing, Jing Hao 
Reproductive BioMedicine Online 
Volume 30, Issue 5, Pages (May 2015)
DOI: /j.rbmo
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2. Figure 1
Spermatogenesis- and oogenesis-specific basic helix-loop-helix (bHLH) transcription factor 2 (Sohlh2) inhibits apoptosis of oocytes. (A) TdT(terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling (TUNEL) results showed that knockdown of Sohlh2 increased apoptotic oocytes. Arrows indicate apoptotic oocytes; (B) TUNEL results showed that overexpression of Sohlh2 decreased apoptotic oocytes; (C) statistical analysis showed, that compared with the corresponding Con group, the difference of apoptotic index of oocytes in the SiSohlh2 group and the P-Sohlh2 group was statistically significant. *P < 0.05 compared with the corresponding group Con. Bars indicate 20 µm.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2
Spermatogenesis- and oogenesis-specific basic helix-loop-helix (bHLH) transcription factor 2 (Sohlh2) upregulates kit ligand (c-kit) expression. (A) Immunocytostaining showed that Sohlh2 knockdown upregulated c-kit expression; (B) semi-quantitative analysis of immunocytostaining results; (C) Western blot results showed that Sohlh2 knockdown downregulated c-kit protein level; (D) semi-quantitative analysis of the Western blot results of C; (E). Western blot results showed that Sohlh2 overexpression upregulated c-kit protein level; (F) semi-quantitative analysis of Western blot results of E; (G) quantitative polymerase chain reaction results of Sohlh2 and c-kit in SiCon and SiSohlh2 group . *P < 0.05 compared with the corresponding group Con. Bars indicate 20 µm.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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4. Figure 3
Spermatogenesis- and oogenesis-specific basic helixloop- helix (bHLH) transcription factor 2 (Sohlh2) knockdown increases nuclear Forkhead box O3 (Foxo3a) and decreases Phosphorylated Foxo3a (P-Foxo3a). (A) Immunocytostaining of Foxo3a showed that Sohlh2 knockdown increased nuclear Foxo3a; (B) semi-quantitative analysis of the immunocytostaining results of A; (C) Western blot results showed that Sohlh2 knockdown decreased P-Foxo3a but not T-Foxo3a; (D) semi-quantitative analysis of the Western blot results of C. *P < Bars indicate 20 µm; (E) Western blot results showed that Sohlh2 knockdown decreased P-Akt1 but not T-Akt1; (F) semi-quantitative analysis of the Western blot results of E. *P < 0.05 compared with the corresponding group Con. Bars indicate 20 µm.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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5. Figure 4
LY blocks spermatogenesis- and oogenesis-specific basic helixloop- helix (bHLH) transcription factor 2 (Sohlh2) effects on oocyte survival via inhibition of phosphorylation of Serine/threonine kinases (Akt) and Forkhead box O3a (Foxo3a). (A) TdT(terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling (TUNEL) results showed that LY ameliorates the function of Sohlh2 on inhibition of oocyte apoptosis. Arrows indicate apoptotic oocytes; (B) semi-quantitative analysis of the TUNEL results of A; (C) Western blot results showed that LY decreased expression of phosphorylation of Foxo3a in the group P-Sohlh2+LY compared with the group P-Sohlh2; (D) semi-quantitative analysis of the Western blot results of C; (E) Western blot results showed that LY decreased phosphorylation of Akt1 in the group P-Sohlh2+LY compared with the group P-Sohlh2; (F) semi-quantitative analysis of the Western blot results of E. *P < 0.05 compared with the corresponding group Con. Bars indicate 20 µm.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions