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Analysis of HER2 Gene Amplification Using an Automated Fluorescence in Situ Hybridization Signal Enumeration System  Rachel Stevens, Imad Almanaseer,

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Presentation on theme: "Analysis of HER2 Gene Amplification Using an Automated Fluorescence in Situ Hybridization Signal Enumeration System  Rachel Stevens, Imad Almanaseer,"— Presentation transcript:

1 Analysis of HER2 Gene Amplification Using an Automated Fluorescence in Situ Hybridization Signal Enumeration System  Rachel Stevens, Imad Almanaseer, Miguel Gonzalez, Derin Caglar, Ryan A. Knudson, Rhett P. Ketterling, Daniel S. Schrock, Thomas A. Seemayer, Julia A. Bridge  The Journal of Molecular Diagnostics  Volume 9, Issue 2, Pages (April 2007) DOI: /jmoldx Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Software placement of nonoverlapping tiles (yellow open squares) in areas with DAPI fluorescence captures the majority of nuclei in each field of view. The Journal of Molecular Diagnostics 2007 9, DOI: ( /jmoldx ) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Two-dimensional per tile HER2/CEP17 spot count distributions. The HER2/CEP17 spot count distribution typical of a homogenous set of cells (all nonamplified or all amplified cells; left) contrasts with that of a mixed population of cells (nonamplified and amplified cells; right). The Journal of Molecular Diagnostics 2007 9, DOI: ( /jmoldx ) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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