1. Smah Abdelhamid

2. Its an analytical techniques that couples chromatographic and spectral methods to exploit the advantages of each Chromatography: identify chemicals purely or nearly purely in complex mixtures. Spectroscopy: yields qualitative information about pure component. Introduce to the scientific community in 1980, by Dr. Tomas Hirschfeld an analytical chemist What?

3. Highly selective Highly sensitive Use in many Applications purifications Polymerization studies Natural products Pharmaceutical Many more…. Why?

5. Very sensitive and selective Most common Sample is injected into injection port of GC, than its vaporized, separated in the GC column, than Analyzed by MS detector, and recorded Provides structural information Volatile compounds Polar compounds GC-MS

6. GC-MS Applications Environomental monitoring and cleanup organic pollutant Criminal forensics Drug detections Fires investigations Explosive investigations Sports Medicine

7. Double three way diverter in line with an auto sampler, and LC system, and the MS Second most common Poor structure determination LC-MS-MS Increase fragmentation LC-UV-MS Biologhical screening for natural products LC-MS

8. LC-MS Applications Pharmacokinetics Bioanalysis Proteomics Metabolomics Drug development

9. Least sensitive High cost Provide best structural information Analysis of natural products Analysis of drug related metabolites in bio-fluids and biomolecules in general analysis of organic molecules Drug impurities Reaction mixtures Drug degradation products HPLC-NMR

10. HPLC-NMR Autosampler, LC pump, Column, a non- NMR detector Flow is guided in the LC-NMR interface Than its guide it to the Flow-cell NMR probe Head Following that the flow is routed to a fraction collector for recovery or further invegistation.

11. CE: Capillary Electrophoresis Analysis is driven by an electric filed in narrow tubes Results in fast separation of compounds (almost all compounds can be separated through this methods) Analysis of purities Assays Analysis of trace level determination CE-MS

12. CE-MS

13. CE-UV GC-FTIR Identifying isomers Ephendrine-type alkaloid found in dietrary supplements containing the chinese herb ma huang HPLC-ESI-MS HPLC-PDA Analysis of coumarins Other Hyphenated Techniques

15. Spectra GC-MSLC-MS

16. Spectra HPLC-NMR CE-MS

17. Conclusion Since the introduction of hyphenated techniques in the 1980s they under went great evolution due to technology, which has expand its use and applications.

18. 1.Patel, K., Patel, J., Patel, M., Rajput, G., & Patel, H. (2010). Introduction to hyphenated techniques and their applications in pharmacy. Pharmaceutical Methods, 2 (1), 2. doi:10.4103/2229-4708.72222 2. The Hy-phen-ated Methods Tomas Hirschfeld Analytical Chemistry 1980 52 (2), 297A-312A 3.http://www.sigmaaldrich.com/analytical- chromatography/analytical-standards/application-area- technique/standards-for-iso-188572.html (images)http://www.sigmaaldrich.com/analytical- chromatography/analytical-standards/application-area- technique/standards-for-iso-188572.html 4.http://www.nature.com/nchembio/journal/v2/n8/fig_ tab/nchembio803_F4.html Reference