1. Dan Zhu, Zu-Hui Wu, Ji-Yao Chen and Lu-Wei Zhou
Effects of electric field on mast cells
Dan Zhu, Zu-Hui Wu, Ji-Yao Chen and Lu-Wei Zhou
Soft-matter Laboratory, State Key Laboratory of Surface Physics, and Department of Physics
Fudan Univiversity, Shanghai, China
Mast cell is a kind of immune cells which is sensitive to physical stimuli such as electric field, however the mechanism of mast cells response to electric field is still not fully understood. This study aims to explore the mechanism of calcium release and activation of TRPV channels stimulated by electric field. A stable AC electric field was applied to mast cells, the cytosol calcium ([Ca2+]c) was measured as well as the corresponding electric field. Calcium oscillation appeared in mast cell when stimulated by AC electric field, the mechanism is to be studied.
I. BACKGROUND
II. METHODOLOGY
Measurements of [Ca2+]i
——confocal fluorescence
microscopy
What is the influence of electric field mast cells ?
IV. Experiment results 2
mast cell
III. Experiment results 1
the peak
2 s
70 s
600s
560 s
530s
500s
450 s
400s
350 s
270 s
210 s
140s
frequence
fluorecence
(concentration of calcium AC 20V 100Hz)
V. Conclusions
Reference
[1] ZuHui Wu , Dan Zhu , JiYao Chen, LuWei Zhou. The effects of thermal Stimulus on Intracellular Calcium Change and Histamine Releases in RBL-2H3 Mast Cells. Journal of Applied Physics In press .
[2] Shaka S. Scarlett, Jody A.White. Regulation of intracellular calcium concentration by nanosecond pulsed electric field. BIOCHIMICA ET BOIPHYSICA ACTA, 1788(2009),
[3] Kyle E. Hammerick, Michael T. Longaker, In vitro effects of direct current eletric field on adipose-derived stromal cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 379(2010), 12-17
1.The concentration of calcium varies in mast cells when stimulated by AC electric field.
2.A model is to be built to simulated the phenomenon .