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Novel diagnostic PCR assay for Burkholderia cenocepacia epidemic strain ST32 and its utility in monitoring infection in cystic fibrosis patients  Klara.

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Presentation on theme: "Novel diagnostic PCR assay for Burkholderia cenocepacia epidemic strain ST32 and its utility in monitoring infection in cystic fibrosis patients  Klara."— Presentation transcript:

1 Novel diagnostic PCR assay for Burkholderia cenocepacia epidemic strain ST32 and its utility in monitoring infection in cystic fibrosis patients  Klara Dedeckova, Lucie Kalferstova, Hynek Strnad, Jolana Vavrova, Pavel Drevinek  Journal of Cystic Fibrosis  Volume 12, Issue 5, Pages (September 2013) DOI: /j.jcf Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

2 Fig. 1 PFGE of SpeI-digested B. cenocepacia ST28, ST33 and ST32 genomes. The strains used were as follows: J2315 (ST28); BCC0560 (ST33; Cardiff University collection), BCC0961 (ST32; Cardiff University collection) and four different ST32 clinical isolates (Prague CF Centre). Journal of Cystic Fibrosis  , DOI: ( /j.jcf ) Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

3 Fig. 2 RAPD fingerprint profiles of B. cenocepacia ST28, ST33 and ST32. Profiles generated using A) RAPD primer 270 and B) RAPD primer 272 [8]. The strains used were as follows: J2315 (ST28); BCC0560 (ST33; Cardiff University collection); BCC0961 (ST32; Cardiff University collection). Journal of Cystic Fibrosis  , DOI: ( /j.jcf ) Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

4 Fig. 3 Characteristics of the DNA region containing the target sequences for ST32-specific PCR assay. White: unique to ST32; grey: common to ST28 and ST32 strains. 1B-t: the PCR target. See Results for a description of parts A to D. Journal of Cystic Fibrosis  , DOI: ( /j.jcf ) Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

5 Fig. 4 Examination strategy for screening and monitoring Bcc infections at the Prague CF Centre. Flow charts applicable to A) new Bcc infections; B) chronic Bcc infections. * If a previous isolate is not available, RAPD is performed for both isolates (the previous and the current one). Journal of Cystic Fibrosis  , DOI: ( /j.jcf ) Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

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