Presentation is loading. Please wait.

Presentation is loading. Please wait.

Expression of the insulin-like growth factor and insulin systems in the luteinizing macaque ovarian follicle  Rebecca S. Brogan, Ph.D., Scott Mix, Muraly.

Published byMaría Luz Alba Camacho Velázquez Modified over 6 years ago

Similar presentations

Presentation on theme: "Expression of the insulin-like growth factor and insulin systems in the luteinizing macaque ovarian follicle  Rebecca S. Brogan, Ph.D., Scott Mix, Muraly."— Presentation transcript:

1 Expression of the insulin-like growth factor and insulin systems in the luteinizing macaque ovarian follicle  Rebecca S. Brogan, Ph.D., Scott Mix, Muraly Puttabyatappa, M.S., Catherine A. VandeVoort, Ph.D., Charles L. Chaffin, Ph.D.  Fertility and Sterility  Volume 93, Issue 5, Pages (March 2010) DOI: /j.fertnstert Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Intrafollicular concentrations of IGF1, IGF2, and insulin during luteinization. IGF1, IGF2, and insulin concentrations were measured using specific RIAs before (0 hours) and 3, 6, 12, and 24 hours after an ovulatory hCG stimulus (n = 5, 5, 5, 4, 5). IGF1 and IGF2 samples were acidified before assay to free IGFs bound to IGFBPs. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Expression of IGF1R, IGF2R, and IR mRNAs during luteinization. Granulosa cells aspirated from rhesus monkeys undergoing controlled ovarian stimulation before (0 hours) and 3, 6, 12, and 24 hours after an ovulatory hCG bolus (n = 5, 5, 4, 3, 3). The mRNA expression of IGF1R (left panel), IGF2R (middle panel), and total IR (right panel) was determined by real-time RT-PCR. Data were normalized to RPL19 mRNA and expressed as relative units. Asterisk indicates significant differences (P<.05) from control (0 hours) samples. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 Granulosa cell expression of IGFBPs during luteinization. Granulosa cells aspirated from rhesus monkeys undergoing controlled ovarian stimulation before (0 hours) and 3, 6, 12, and 24 hours after an ovulatory hCG bolus (n = 5, 5, 4, 3, 3). The mRNA expression of IGFBP2 (top left panel), IGFBP3 (top right), IGFBP4 (middle left), IGFBP5 (middle right), IGFBP6 (lower left), and PAPP-A (lower right) was determined by real-time RT-PCR. Data were normalized to RPL19 mRNA and expressed as relative units. Asterisk indicates significant differences (P<.05) from control (0 hours) samples. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

5 Figure 4 Follicular fluid levels of IGFBPs and PAPP-A during luteinization. Follicular fluid was collected from rhesus monkeys undergoing controlled ovarian stimulation before (0 hours) and 3, 6, 12, and 24 hours after an ovulatory hCG bolus (n = 3, 3, 2, 3, 5). (A) Representative example of a Western blot showing the IGFBP2 bands during the time course of hCG treatment (top panel) normalized to the large proteins remaining in the gel post-transfer (bottom panel). (B). The follicular fluid protein profiles of IGFBP2–6 are shown in graphical form. The Western blots were completed, membranes and gels scanned into a PDF file, and IGFBP band density normalized to coomassie blue-stained protein using the program U-SCAN IT (Silk-Scientific Corporation). Asterisk indicates significant differences (P<.05) from control (0 hours) samples. The hatch mark (#) indicated significant differences between 3 and 6 hours of hCG treatment (IGFBP4). (C) follicular fluid levels of PAPP-A determined by ELISA. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Download ppt "Expression of the insulin-like growth factor and insulin systems in the luteinizing macaque ovarian follicle  Rebecca S. Brogan, Ph.D., Scott Mix, Muraly."

Similar presentations

Reduction of estrogen production by interleukin-6 in a human granulosa tumor cell line may have implications for endometriosis-associated infertility 

Reduction of estrogen production by interleukin-6 in a human granulosa tumor cell line may have implications for endometriosis-associated infertility 
Human chorionic gonadotropin controls luteal vascular permeability via vascular endothelial growth factor by down-regulation of a cascade of adhesion.

Human chorionic gonadotropin controls luteal vascular permeability via vascular endothelial growth factor by down-regulation of a cascade of adhesion.
Role of cAMP-responsive element modulator-τ (CREMτ) in ipsilateral testicular injury after unilateral testicular torsion-detorsion  Si-Ming Wei, M.D.,

Role of cAMP-responsive element modulator-τ (CREMτ) in ipsilateral testicular injury after unilateral testicular torsion-detorsion  Si-Ming Wei, M.D.,
Curcumin attenuates ischemia–reperfusion injury in rat testis

Curcumin attenuates ischemia–reperfusion injury in rat testis
Vascular endothelial growth factor A and its two receptors in human preantral follicles from fetuses, girls, and women  Ronit Abir, Ph.D., Asangla Ao,

Vascular endothelial growth factor A and its two receptors in human preantral follicles from fetuses, girls, and women  Ronit Abir, Ph.D., Asangla Ao,
Shalmali J. Dharma, M. Sc. , Deepak N. Modi, Ph. D. , Tarala D

Shalmali J. Dharma, M. Sc. , Deepak N. Modi, Ph. D. , Tarala D
Rong Hu, Ph. D. , Fei-miao Wang, M. D. , Liang Yu, Ph. D. , Yan Luo, M

Rong Hu, Ph. D. , Fei-miao Wang, M. D. , Liang Yu, Ph. D. , Yan Luo, M
Developmental programming: prenatal testosterone excess disrupts anti-Müllerian hormone expression in preantral and antral follicles  Almudena Veiga-Lopez,

Developmental programming: prenatal testosterone excess disrupts anti-Müllerian hormone expression in preantral and antral follicles  Almudena Veiga-Lopez,
Selective impairment in glycogen synthase kinase-3 and mitogen-activated protein kinase phosphorylation: comparisons with the hyperandrogenic and the.

Selective impairment in glycogen synthase kinase-3 and mitogen-activated protein kinase phosphorylation: comparisons with the hyperandrogenic and the.
Effective treatment of experimental human endometrial cancers with targeted cytotoxic luteinizing hormone–releasing hormone analogues AN-152 and AN-207 

Effective treatment of experimental human endometrial cancers with targeted cytotoxic luteinizing hormone–releasing hormone analogues AN-152 and AN-207 
Adiponectin increases insulin-like growth factor I-induced progesterone and estradiol secretion in human granulosa cells  Christine Chabrolle, M.D., Lucie.

Adiponectin increases insulin-like growth factor I-induced progesterone and estradiol secretion in human granulosa cells  Christine Chabrolle, M.D., Lucie.
Sphingosine-1-phosphate inhibits H2O2-induced granulosa cell apoptosis via the PI3K/Akt signaling pathway  Tatsuo Nakahara, M.D., Akira Iwase, M.D., Ph.D.,

Sphingosine-1-phosphate inhibits H2O2-induced granulosa cell apoptosis via the PI3K/Akt signaling pathway  Tatsuo Nakahara, M.D., Akira Iwase, M.D., Ph.D.,
Daily physical activity and hot flashes in the Study of Women's Health Across the Nation (SWAN) Flashes Study  Carolyn Gibson, M.P.H., M.S., Karen Matthews,

Daily physical activity and hot flashes in the Study of Women's Health Across the Nation (SWAN) Flashes Study  Carolyn Gibson, M.P.H., M.S., Karen Matthews,
Resistin decreases insulin-like growth factor I–induced steroid production and insulin- like growth factor I receptor signaling in human granulosa cells 

Resistin decreases insulin-like growth factor I–induced steroid production and insulin- like growth factor I receptor signaling in human granulosa cells 
MRNA expression pattern of insulin-like growth factor components of granulosa cells and cumulus cells in women with and without polycystic ovary syndrome.

MRNA expression pattern of insulin-like growth factor components of granulosa cells and cumulus cells in women with and without polycystic ovary syndrome.
The human ovarian follicular fluid level of interleukin-8 is associated with follicular size and patient age  Beth A. Malizia, M.D., Yoo Sang Wook, M.D.,

The human ovarian follicular fluid level of interleukin-8 is associated with follicular size and patient age  Beth A. Malizia, M.D., Yoo Sang Wook, M.D.,
Regulation of vascular endothelial growth factor-A and its soluble receptor sFlt-1 by luteinizing hormone in vivo: implication for ovarian follicle angiogenesis 

Regulation of vascular endothelial growth factor-A and its soluble receptor sFlt-1 by luteinizing hormone in vivo: implication for ovarian follicle angiogenesis 
The impact of culture conditions on early follicle recruitment and growth from human ovarian cortex biopsies in vitro  Jana Liebenthron, M.Sc., Maria.

The impact of culture conditions on early follicle recruitment and growth from human ovarian cortex biopsies in vitro  Jana Liebenthron, M.Sc., Maria.
Network analyses of differentially expressed proteins in amniotic fluid supernatant associated with abnormal human karyotypes  Tzu-Hao Wang, M.D., Ph.D.,

Network analyses of differentially expressed proteins in amniotic fluid supernatant associated with abnormal human karyotypes  Tzu-Hao Wang, M.D., Ph.D.,
2-Methoxyestradiol in the human corpus luteum throughout the luteal phase and its influence on lutein cell steroidogenesis and angiogenic activity  Paulina.

2-Methoxyestradiol in the human corpus luteum throughout the luteal phase and its influence on lutein cell steroidogenesis and angiogenic activity  Paulina.

Similar presentations

Ads by Google