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Tranilast, an orally active antiallergic compound, inhibits extracellular matrix production in human uterine leiomyoma and myometrial cells  Md Soriful.

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Presentation on theme: "Tranilast, an orally active antiallergic compound, inhibits extracellular matrix production in human uterine leiomyoma and myometrial cells  Md Soriful."— Presentation transcript:

1 Tranilast, an orally active antiallergic compound, inhibits extracellular matrix production in human uterine leiomyoma and myometrial cells  Md Soriful Islam, Ph.D., Olga Protic, M.Sc., Andrea Ciavattini, M.D., Stefano Raffaele Giannubilo, M.D., Andrea Luigi Tranquilli, M.D., William H. Catherino, M.D., Ph.D., Mario Castellucci, M.D., Ph.D., Pasquapina Ciarmela, Ph.D.  Fertility and Sterility  Volume 102, Issue 2, Pages (August 2014) DOI: /j.fertnstert Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 (A) Immunocytochemical staining of α-SMA in human primary myometrial and leiomyoma cells. (B–D) Real-time PCR of fibronectin, collagen 1A1, and versican in human primary myometrial and leiomyoma cells. Results are the mean ± SEM of three experiments performed using independent cell cultures from different tissues. Each experiment was done in triplicate. ∗P<.05. NT = untreated cells. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 (A–C) Real-time PCR of fibronectin, collagen 1A1, and versican in leiomyoma cell line. Experiments were performed twice, and no statistically significant difference was observed between experiments. Values are expressed as the mean of six replications from independent experiment ± SEM. ∗P<.05. NT = untreated cells. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 (A, B) Immunhistochemical staining of fibronectin and collagen 1 in human myometrial and leiomyoma tissue. (C–F) Immunocytochemical staining of fibronectin and collagen 1 in human primary myometrial and leiomyoma cells. Results are the mean ± SEM of three experiments performed using independent cell cultures from different tissues. Each experiment was done in triplicate. ∗P<.05. NT = untreated cells. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

5 Figure 3 (A, B) Immunhistochemical staining of fibronectin and collagen 1 in human myometrial and leiomyoma tissue. (C–F) Immunocytochemical staining of fibronectin and collagen 1 in human primary myometrial and leiomyoma cells. Results are the mean ± SEM of three experiments performed using independent cell cultures from different tissues. Each experiment was done in triplicate. ∗P<.05. NT = untreated cells. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

6 Figure 4 (A, B) Real-time PCR of activin-A in human primary myometrial and leiomyoma cells. Results are the mean ± SEM of three experiments performed using independent cell cultures from different tissues. Each experiment was done in triplicate. ∗P<.05. NT = untreated cells. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions


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