1. Expression of leptin receptor mRNA in cumulus cells is correlated with expression of PTX3 
Helena T.A. van Tol, Johannes C.M. Vernooij, Ben Colenbrander, Dagmar Gutknecht, Nicholas S. Macklon, Bernard A.J. Roelen 
Reproductive BioMedicine Online 
Volume 20, Issue 6, Pages (June 2010)
DOI: /j.rbmo
Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

2. Figure 1
Follicular-fluid concentration of (A) leptin, (B) soluble leptin receptor (solObR) and (C) free leptin index as a function of body mass index (BMI). BMI <20kg/m2, n=10; BMI 20–25kg/m2, n=31; BMI >25kg/m2, n=16), values are means±SEM. Data were logarithmically transformed (LN) before analysis. Bars with different superscripts are significantly different as analysed by one-way ANOVA (P<0.05).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2
Expression of (A) total leptin receptor (ObR-TOT) and (B) short isomer of leptin receptor (ObR-Short) in four representative samples of per-patient pooled human cumulus cells as detected with quantitative reverse-transcription PCR after 35 cycles of amplification. Expression of long isomer of leptin receptor (ObR-Long) in the same samples after (C) 35 and (D) 40 cycles. Expression levels of ObR-Long in sample 1 and 2 were too low to quantify. L=100bp DNA marker; pos=positive control; neg=negative control.
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4. Figure 3
Average expression stability values M of candidate genes after stepwise exclusion of the least stable gene. Genes are ranked according to increasing expression stability.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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5. Figure 4
Correlation of gene expression levels between (A, B, and C) leptin receptor (ObR) isoforms and between (D and E) total ObR isoforms (ObR-TOT), short ObR isoform (ObR-Short) and PTX3 in human cumulus cells after maturation. (F) No correlation was found between expression levels of long ObR isomer (ObR-Long) and PTX3. Data were logarithmically transformed before analysis. NS=not statistically significant; rel.=relative.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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6. Figure 5
Correlation between the number of oocytes retrieved after puncture and the gene expression levels of (A) total leptin receptor (ObR-TOT) and (B) short isoform of ObR (ObR-Short) in human cumulus cells. (C) No significant correlation was found between ObR-TOT and the normalization factor. Data concerning expression levels were logarithmically transformed before analysis. rel.=relative; NS=not statistically significant.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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7. Figure 6
Women with follicular-fluid leptin concentrations ⩽0.5ng/mg protein had a significantly higher incidence of an ongoing pregnancy after IVF/intracytoplasmic sperm injection treatment compared with women with leptin concentration >0.5ng/mg protein. The number of women per group is indicated in brackets. ∗P<0.05.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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