1. Mass Spectrometry

2. Analytical Chemistry • Chromatography (Separations)
• Mass Spectrometry
• Infrared (IR) Spectroscopy
• Nuclear Magnetic Resonance (NMR) Spectroscopy
• X-ray Crystallography (visual solid state molecular structure)

3. Mass Spectrometry What insights are we hoping to gain?
• Molecular Mass/Isotopic Distribution
• Molecular Formula
• Some Structural Characteristics – Fragmentation Pattern

4. Mass Spectromeric Isotope Indentification
Only molecules of the correct m/z (mass/charge) will make it to the detector.

5. EI-MS of toluene

6. EI-MS of 2,2-Dimethyl Pentane

7. EI-MS of 2,2-Dimethyl Pentane
Peaks with smaller m/z values __ called fragment ion peaks __ represent positively charged fragments of the parent molecule

8. EI-MS of 2,2-Dimethyl Pentane

9. EI-MS of n-Propanol

10. EI-MS of 4-Heptanone

11. EI-MS of 4-Heptanone

12. EI-MS of 4-Heptanone

13. Isotopes

14. EI-MS of Benzyl Bromide

15. Isotopes

16. EI-MS of Benzyl Bromide

17. EI-MS of 4-Chloroanisole

18. Isotopes

19. Isotopes