1. Volume 121, Issue 6, Pages 1400-1406 (December 2001)
D-glucose releases 5-hydroxytryptamine from human BON cells as a model of enterochromaffin cells 
Minsoo Kim, Helen J. Cooke, Najma H. Javed, Hannah V. Carey, Fievos Christofi, Helen E. Raybould 
Gastroenterology 
Volume 121, Issue 6, Pages (December 2001)
DOI: /gast
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2. Fig. 1
Effect of 50 μmol/L brefeldin A (BFA) on basal and 10 μmol/L forskolin-stimulated 5-HT release expressed as percent change from baseline. *P < 0.05 vs. zero control; n = 3.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

3. Fig. 2
Effect of increasing concentrations of D-glucose and mannitol on 5-HT release expressed as percent change from baseline. (A) Noncloned BON cells. Basal 5-HT release was 0.86 ± 0.07 pmol/well per 20 minutes; n = 5. (B) Subcloned BON cells. Basal 5-HT release was 0.56 ± 0.15 pmol/well per 20 minutes; n = 3–4. *P < 0.05 vs. zero control.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Comparison of nonmetabolizable αMG with other stimulants of 5-HT release. GLU, glucose; MAN, mannitol; ISO, isoproterenol. 5-HT release is expressed as percent change from baseline. BON0 cells were treated with 75 mmol/L αMG, GLU, or MAN, and 0.1 mmol/L ISO was used as a positive control. Baseline 5-HT release was 0.73 ± 0.06 pmol/well per 20 minutes; n = 7–9. *P < 0.05 vs. zero controls.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

5. Fig. 4
Effect of 0.1 mmol/L phloridzin on 75 mmol/L glucose (GLU)– and 75 mmol/L αMG–stimulated 5-HT release expressed as percent change from baseline. Basal.5-HT release was 0.41 ± 0.04 pmol/well per 20 minutes; n = 4–11. *P < 0.05 vehicle vs. phloridzin.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

6. Fig. 5
Comparison of the effects of 50 mmol/L D-glucose (GLU), 50 mmol/L galactose (GAL), and 50 mmol/L fructose (FRU) on 5-HT release from BON7 cells, expressed as percent change from baseline. Basal 5-HT release was 1.2 ± 0.16 pmol/well per 20 minutes; n = 4–10. *P < 0.05.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

7. Fig. 6
cDNA amplified by polymerase chain reaction. Reverse-transcribed cDNA from HEK293 cells was a positive control (HEK). For negative control, water was substituted for the cDNA (H2O). The 208-bp polymerase chain reaction product (arrow) from the BON cell was excised, purified, and sequenced.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

8. Fig. 7
Western blotting of SGLT1, GLUT2, and GLUT5. Protein preparations from human jejunum and BON cells were solubilized in sample buffer and separated by 10%–12.5% sodium dodecyl sulfate–polyacrylamide gel electrophoresis. Proteins were transferred to the nitrocellulose membrane. (A) Membrane probing with polyclonal anti-SGLT1 antibody (Ab) indicated the presence of a specific bands of 55–60 kilodaltons in both human jejunum and BON cell preparations. Preabsorption of anti-SGLT1 antibody with immunogenic blocking peptide prevented appearance of the band. (B) Membrane probing with polyclonal anti-GLUT2 and anti-GLUT5 antibodies revealed specific bands of 47–50 kilodaltons and 45 kilodaltons, respectively, in human jejunum and BON cells.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions