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Volume 123, Issue 3, Pages (September 2002)

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Presentation on theme: "Volume 123, Issue 3, Pages (September 2002)"— Presentation transcript:

1 Volume 123, Issue 3, Pages 810-816 (September 2002)
The type of sodium-coupled solute modulates small bowel mucosal injury, transport function, and ATP after ischemia/reperfusion injury in rats  Rosemary A. Kozar, Stanley G. Schultz‡, Heitham T. Hassoun, Roland Desoignie, Norman W. Weisbrodt, Marian M. Haber, Frederick A. Moore  Gastroenterology  Volume 123, Issue 3, Pages (September 2002) DOI: /gast Copyright © 2002 American Gastroenterological Association Terms and Conditions

2 Fig. 1 Gut absorptive capacity after solute administration during gut I/R. Jejunum was mounted in a Ussing chamber and gut absorptive capacity was assessed by measuring the change in Isc after the addition of 10 mmol/L glucose to the mucosal reservoir and 10 mmol/L mannitol to the serosal reservoir (osmotic control). Values are expressed as means ± SEM, n = 6–9. Means with different letters (a or b) are significantly different. Sham control, sham laparotomy with mannitol; Sham glu/ala, sham laparotomy with (glucose + alanine); I/R control, I/R with mannitol; I/R glu/ala, I/R with (glucose + alanine); I/R Glu, I/R with glucose; I/R Ala, I/R with alanine; and I/R glut, I/R with glutamine. Gastroenterology  , DOI: ( /gast ) Copyright © 2002 American Gastroenterological Association Terms and Conditions

3 Fig. 2 Representative short-circuit tracings comparing the change in Isc over time after glucose stimulation. Long arrow, addition of glucose; short arrow, addition of phloridzin. ●, Sham; ▴, I/R control; ■, I/R alanine; ▾, I/R glucose; ♦, I/R glutamine; (hex symbol), I/R glucose + alanine. Gastroenterology  , DOI: ( /gast ) Copyright © 2002 American Gastroenterological Association Terms and Conditions

4 Fig. 3 Theophylline stimulation after solute administration during gut I/R. Jejunum was mounted in an Ussing chamber and the response to theophylline was assessed by measuring the change in Isc after the addition of 50 mmol/L theophylline to the mucosal and serosal reservoirs. Values are expressed as means ± SEM, n = 6–9. Means with different letters (a or b) are significantly different. Sham control, sham laparotomy with mannitol; Sham glu/ala, sham laparotomy with (glucose + alanine); I/R control, I/R with mannitol; I/R glu/ala, I/R with (glucose + alanine); I/R Glu, I/R with glucose; I/R Ala, I/R with alanine; and I/R glut, I/R with glutamine. Gastroenterology  , DOI: ( /gast ) Copyright © 2002 American Gastroenterological Association Terms and Conditions

5 Fig. 4 Representative photomicrographs of jejunal mucosa after: (A and B) sham, Chiu 0; (C and D) I/R glucose, Chiu 3; and (E and F) I/R alanine, Chiu 5 (A, C, and E at 10× and B, D, and F at 20×). Sham, sham laparotomy with (glucose + alanine); I/R glucose, I/R with glucose; and I/R alanine, I/R with alanine. Gastroenterology  , DOI: ( /gast ) Copyright © 2002 American Gastroenterological Association Terms and Conditions


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