1. Plasmodium Detection and Differentiation by Direct-on-Blood PCR Nucleic Acid Lateral Flow Immunoassay 
Johanna M. Roth, Laura de Bes, Patrick Sawa, George Omweri, Victor Osoti, Boris Oberheitmann, Henk D.F.H. Schallig, Pètra F. Mens 
The Journal of Molecular Diagnostics 
Volume 20, Issue 1, Pages (January 2018)
DOI: /j.jmoldx
Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
Possible test outcomes of the pan-Plasmodium/Plasmodium falciparum (pan/P. falciparum) (A) and pan-Plasmodium/Plasmodium vivax (pan/P. vivax) (B) direct-on-blood PCR nucleic acid lateral flow immunoassay (db-PCR-NALFIA). A: 1. The pan line (left) and the control line are visible. The test result is Plasmodium positive, but the infecting agent is not P. falciparum. 2. The pan line, P. falciparum line, and control line are positive. The sample contains P. falciparum. 3. Only a control line is visible. The test result is negative for Plasmodium. 4. Control and test lines are absent: water control or invalid test. B: 1. The pan line (left) and the control line are visible. The test result is Plasmodium positive, but the infecting agent is not P. vivax. 2. The pan line, P. vivax line, and control line are positive. The sample contains P. vivax. 3. Only a control line is visible. The test result is negative for Plasmodium. 4. Control and test lines are absent: invalid test or water control.
The Journal of Molecular Diagnostics  , 78-86DOI: ( /j.jmoldx )
Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
Description of patient flow. db-PCR-NALFIA, direct-on-blood PCR nucleic acid lateral flow immunoassay; qPCR, real-time quantitative PCR.
The Journal of Molecular Diagnostics  , 78-86DOI: ( /j.jmoldx )
Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions