1. Volume 149, Issue 6, Pages 1519-1529 (November 2015)
Deficiency in Lysophosphatidylcholine Acyltransferase 3 Reduces Plasma Levels of Lipids by Reducing Lipid Absorption in Mice 
Zhiqiang Li, Hui Jiang, Tingbo Ding, Caixia Lou, Hai H. Bui, Ming-Shang Kuo, Xian-Cheng Jiang 
Gastroenterology 
Volume 149, Issue 6, Pages (November 2015)
DOI: /j.gastro
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2. Figure 1
Generation and characterization of LPCAT3 KO mouse. (A) Strategy used to disrupt mouse Lpcat 3 gene. (B) LPCAT3 mRNA in small intestine and liver from 2-week-old WT and LPCAT3 KO mice. (C) Total cholesterol, phospholipid, and triglyceride levels in the plasma from 10-day-old WT and LPCAT3 KO mice. (D) Small intestine Oil Red O staining from 10-day-old mice. (E) Small intestine NPC1L1 immunofluorescence staining from 10-day-old mice. (F) Small intestine FATP4 immunofluorescence staining from 10-day-old mice. FATP4 signal was indicated by red arrows. Values are mean ± SD, n = 5; ∗P < .05.
Gastroenterology  , DOI: ( /j.gastro )
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3. Figure 2
The comparison between LPCAT3 KO and WT mice. LPCAT3 KO mice (both male and female) were rescued with PC/olive oil. (A) Body weight comparison. LPCAT3 KO mice indicated by red arrows. (B, C) Small intestine length comparison. (D) Diameter comparison. (E) Villi comparison. Wider villi were indicated by red arrowheads. Values are mean ± SD, n = 5; ∗P < .05.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2015 AGA Institute Terms and Conditions

4. Figure 3
Measurements of total LPCAT activity and plasma lipoprotein levels in WT and LPCAT3 KO mice. (A) Total LPCAT activity in small intestine and liver of WT and LPCAT3 KO male mice, measured as described in Materials and Methods. (B) Total cholesterol, phospholipid, and triglyceride levels in plasma. (C) Fluorogram of plasma apolipoprotein levels by Western blotting. Plasma (0.2 μL) was separated by 4%−15% sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotted with polyclonal antibodies against apoA-I, apoB, apoE, and albumin. (D) Quantitation of plasma apoA-I, apoB, and apoE levels. (E) Plasma lipoprotein distribution by fast protein liquid chromatography. Total cholesterol, phospholipids, and triglycerides were determined in each fraction. Values are mean ± SD, n = 5; ∗P < .05.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2015 AGA Institute Terms and Conditions

5. Figure 4
Triglyceride and cholesterol absorption in LPCAT3 KO and WT mice. (A) and (B) male mice, at 10 weeks old, were gavaged with 0.2 μCi [3H]triolein and 0.1 μCi [14C]cholesterol in 15 μL olive oil. Blood was collected over 24 hours and measured for the presence of [3H]glycerolipids and [14C]cholesterol. (C) Mice were gavaged with 0.1 μCi [14C]cholesterol and 0.2 μCi [3H]sitostanol in 15 μL olive oil. Feces were collected for 48 hours and lipids were extracted for counting. (D) Mice (male) were fed a high-fat/cholesterol diet for 10 days, then feces were collected for 2 days and lipids were extracted to determine total cholesterol, triglyceride, and phospholipid levels. (E, F) Western blots fluorogram and quantitation of NPC1L1, CD36, FATP4, ABCG8, ABCA1, and MTP in enterocyte homogenates from LPCAT3 KO and WT small intestine. β-Actin was used as a loading control. Values are mean ± SD, n = 4; ∗P < .05.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2015 AGA Institute Terms and Conditions

6. Figure 5
Measurement of NPC1L1 and FATP4 protein levels in 10-week-old LPCAT3 KO and WT mice. (A) Small intestine NPC1L1 immunostaining. (B) Small intestine FATP4 immunostaining. (C, D) Western blot fluorogram and quantitation of NPC1L1 in lipid rafts fractions from primary enterocytes. Lyn kinase was used as marker for lipid rafts and non-rafts. Values are mean ± SD, n = 4; ∗P < .01.
Gastroenterology  , DOI: ( /j.gastro )
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7. Figure 6
Western blot of ABCA1, SR-BI, and MTP in liver homogenates, as well as lipoprotein production in vivo. (A) Fluorogram of liver ABCA1, SR-BI, MTP, and β-actin (loading control). (B) Quantitation of ABCA1, SR-BI, and MTP in the liver. (C) In vivo VLDL and HDL production measurement. Fluorogram of plasma 35S-apoB and 35S-apoA-I on VLDL and HDL, respectively. (D) Quantitation of 35S-apoB and 35S-apoA-I in blood. Values are mean ± SD, n = 4−5; ∗P < .05.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2015 AGA Institute Terms and Conditions