1. Bacteroidales LPS inhibits TLR stimulation.
Bacteroidales LPS inhibits TLR stimulation. (A to C) LPS was isolated from the species indicated. (A) Human PBMCs were stimulated in the presence of increasing amounts of LPS from the Bacteroidales species indicated. IL-6, TNF-α, and IL-1β concentrations in supernatants were measured after 20 h of culture. E. coli LPS was included as a control. Data shown are the mean ± the standard deviation of triplicates in one representative experiment. (B and C) Human PBMCs were cotreated with E. coli LPS and increasing doses of LPS from Bacteroidales (B) and Alistipes (C) spp. TNF-α and IL-1β concentrations in supernatants were measured after 20 h of culture. E. coli LPS is shown as a control (dotted line). Data shown are the mean + the standard deviation of triplicate measurements in one representative of three or more independent experiments. *, corrected P value of <0.05. (C) MALDI-TOF MS analysis of the lipid A moiety of individual Bacteroidales and E. coli LPSs. m/z values are indicated for major peaks. On the right are the lipid A structures associated with the main m/z values.
Eva d’Hennezel et al. mSystems 2017; doi: /mSystems