1. Effects of Tetracycline and Kanamycin on Pseudomonas fluorescens
Naomi Drake, Mary Powell, Alexis County

2. Low Down
Question: Which antibiotic, Tetracycline or Kanamycin, has the greatest effect on the lag phase of Pseudomonas fluorescens?
Hypothesis: The organism’s lag phase would be more significantly extended in the presence of kanamycin as opposed tetracycline.

3. Note: Dilutions 1:10 and 1:100 fully inhibited growth
Initial growth: Dilution 1:10,000- reached .4 9:45:05 hours [.392, 10:00:05 hours]
Extended Lag phase: Dilution 1:1000- reached .4 hours [.394, 20:15:05 hours]
Note: Dilutions 1:10 and 1:100 fully inhibited growth

4. Initial growth: Control (no antibiotic)- reached 0
Initial growth: Control (no antibiotic)- reached :30:05 hours [.397 OD, :45:05 hours]
Extended Lag Phase: Dilution 1:1600 – reached :30:05 hours [.398 OD, .412 hours]

5. Tetracycline vs Kanamycin

6. Conclusions
Based on the data collected, P. fluorescens is resistant to tetracycline in various dilutions ranging from 1:10 to 1:12,800.
In the presence of Kanamycin however, the bacteria was susceptible, in varying ratios, ranging from dilutions 1:2 to 1:12,800.