1. Biotechnology and Recombinant DNA
Chapter 9, part B
Biotechnology and Recombinant DNA

2. Genetic Engineering
Figure

3. Genetic Engineering
Figure

4. Making a Gene Product
Figure

5. Making a Gene Product
Figure

6. E. coli Used because it is easily grown and its genomics are known
Need to eliminate endotoxin from products
Cells must be lysed to get product

7. Making a Gene Product
Figure 9.13

8. Therapeutic Applications
Subunit vaccines
Nonpathogenic viruses carrying genes for pathogen's antigens as vaccines
Gene therapy to replace defective or missing genes
Human Genome Project
Nucleotides have been sequenced
Human Proteome Project may provide diagnostics and treatments

9. Random Shotgun Sequencing
Figure 9.14

10. Scientific Applications
Understanding of DNA
Sequencing organisms' genomes
DNA fingerprinting for identification
Figure 9.16

11. Southern Blotting
Figure

12. Southern Blotting
Figure

13. Southern Blotting
Figure

14. Agricultural Applications
Table 9.2

15. Genetic Engineering Using Agrobacterium
Figure 9.18

16. Safety Issues and Ethics
Avoid accidental release
Genetically modified crops must be safe for consumption and for the environment
Who will have access to an individual's genetic information?