1. Expression of the Human Immunodeficiency Virus-Tat Gene in Lymphoid Tissues of Transgenic Mice Is Associated With B-Cell Lymphoma
by Ramendra K. Kundu, Frank Sangiorgi, Lan-Ying Wu, Paul K. Pattengale, David R. Hinton, Parkash S. Gill, and Robert Maxson
Blood
Volume 94(1):
July 1, 1999
©1999 by American Society of Hematology

2. Schematic diagram showing the structure of the β-actin–Tat transgene.
Schematic diagram showing the structure of the β-actin–Tat transgene. The Tat cDNA was placed under the transcriptional control of the chicken β-actin promoter. Transcriptional splicing and termination functions were provided by an SV40-derived 3′ UTR and polyadenylation signal. (▩), Chicken β-actin promoter; (▩), Tat cDNA.
Ramendra K. Kundu et al. Blood 1999;94:
©1999 by American Society of Hematology

3. Ramendra K. Kundu et al. Blood 1999;94:275-282
©1999 by American Society of Hematology

4. Ramendra K. Kundu et al. Blood 1999;94:275-282
©1999 by American Society of Hematology

5. Histological analysis of spleens of Tat-transgenic and control mice.
Histological analysis of spleens of Tat-transgenic and control mice. Tat-transgenic mice (lines 21 and 29) and littermate control mice were killed at 12 to 15 months of age. Spleens were removed and processed for histology. Shown are the representative hematoxylin/eosin stains of (A) a control spleen (10×), (B) a Tat-transgenic spleen (10×), (C) a Tat-transgenic spleen at high magnification (25×).
Ramendra K. Kundu et al. Blood 1999;94:
©1999 by American Society of Hematology

6. Southern blot showing Ig heavy-chain rearrangement in splenic DNA of Tat-transgenic mice.
Southern blot showing Ig heavy-chain rearrangement in splenic DNA of Tat-transgenic mice. DNA was extracted from spleens of two independent lines of Tat-transgenic mice, 24 and 29. The DNA was digested with EcoRI for IgJ heavy-chain analysis orHpaI for TCR-β analysis, blotted, and hybridized with an (A) IgJ heavy-chain probe or (B) TCR-β probe. C, control mouse; T, Tat-transgenic mouse. C1-C3 and T1-T3 refer to individual control and transgenic mice. Transgenic lines from which the mice were derived are indicated above the blots. The sizes of the bands in kilobase are indicated on the left. The 2.2-kb, 2.5-kb, and 3.1-kb bands in the Tat-transgenic samples indicate a JH rearrangement; there was no apparent rearrangement of the TCR-β gene.
Ramendra K. Kundu et al. Blood 1999;94:
©1999 by American Society of Hematology

7. Ramendra K. Kundu et al. Blood 1999;94:275-282
©1999 by American Society of Hematology

8. Ramendra K. Kundu et al. Blood 1999;94:275-282
©1999 by American Society of Hematology