1. Volume 67, Issue 1, Pages 7-10 (January 2015)
MicroRNA Expression Profile of Primary Prostate Cancer Stem Cells as a Source of Biomarkers and Therapeutic Targets 
Jayant K. Rane, Mauro Scaravilli, Antti Ylipää, Davide Pellacani, Vincent M. Mann, Matthew S. Simms, Matti Nykter, Anne T. Collins, Tapio Visakorpi, Norman J. Maitland 
European Urology 
Volume 67, Issue 1, Pages 7-10 (January 2015)
DOI: /j.eururo
Copyright © 2014 European Association of Urology Terms and Conditions

2. Fig. 1
Cell subtype, rather than pathologic status, is a primary determinant of microRNA (miRNA) expression. (a) A schematic of human prostate epithelial hierarchy (left) showing a stemlike cell with a basal phenotype subsequently differentiating into luminal cells by way of committed basal (CB) cells. The subpopulations were enriched from normal human prostate epithelial cells, benign prostatic hyperplasia (BPH), and cancers (high Gleason grade, treatment-naive prostate cancer [PCa], and castration-resistant PCa [CRPC]). Principal component analysis was performed on miRNA microarray profiles of cultured stem cells (SCs) and CB cells at passage 2 (right). (b) Comparison of miRNA expression profiles for unfractionated CRPC tissue (vs BPH) [8] and prostate SC (vs CB). (c) Kaplan-Meier curve for PCa patient survival with differential miR-548c-3p expression using Taylor et al. [12]. (d) Colony-forming efficiency of miR-548c-3p transfected CB cells (n=3 for BPH, n=3 for PCa; each sample in triplicate). (e) Fluorescence-activated cell sorting analysis for CD49b (integrin β2) and CD49f (integrin β6) expression performed on CB cells transfected with either control or miR-548c-3p for 3 d (n=3 for BPH, n=3 for PCa; each sample in triplicate). (f) Live cell count of miR-548c-3p transfected CB cells 48h after exposure to 5-Gy radiation (n=3 for BPH, n=3 for PCa; each sample in triplicate). (g) Quantitative reverse transcription polymerase chain reaction analysis for miR-548c-3p expression in epithelial cells enriched from freshly disaggregated uncultured BPH tissue (n=3), PCa Gleason grade 7 tissue (n=5), and CRPC tissue (n=3). Each sample was assessed in triplicate. Data are expressed as mean plus or minus standard deviation. * p<0.05 (student t test); ** p<0.01 (student t test); *** p<0.001 (student t test).
BPH=benign prostatic hyperplasia; CFE=colony-forming efficiency; CRPC=castration-resistant prostate cancer; miRNA=microRNA; PCa=prostate cancer; PREC=prostate epithelial cell.
European Urology  , 7-10DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions