1. Welcome To The Centre for Applied Genomics

2. Quotable Quotes
"The scientist is not a person who gives the right answers;
he's one who asks the right questions.
~ Claude Lévi-Strauss

3. This week at TCAG Lab meeting
"Explosive factors and exciting mechanisms controlling allele preference in imprinting and gene expression"
Given by Layla Parker-Katiraee
Wednesday, Nov 14, 1:30 PM
14th Floor Conference Room

5. Oligo synthesis 17,946 oligos synthesized since January 1, 2006
up to 120 bases
conventional & modified
free delivery to downtown Toronto
New lower prices!
40 nmole 200 nmole 1000 nmole
Cost per base $ $ $1.89
Cartridge purification $ $ $30.00
17,946 oligos synthesized
since January 1, 2006

6. DNA Sequencing 146,750 reads since January 1, 2006
plasmids, PCR products
BAC ends, other genomic clones
mutation screening
Solexa next-generation sequencing
146,750 reads since January 1, 2006

7. Now available! Ultra-high throughput sequencing
with the Illumina Genome Analyzer

8. Biobanking 7,182 cell lines handled since January 1, 2006
DNA preparation
cell line transformation
DNA, white cell, and cell line banking
other cells on request
7,182 cell lines handled since January 1, 2006

9. Genome resources 5,218 clones prepared since January 1, 2006
cDNA libraries
genomic clone libraries
whole-genome DNA amplification
project support – database queries, annotation, probe selection
5,218 clones prepared since January 1, 2006

10. Genetic analysis > 2.5 billion genotypes since January 1, 2006
TaqMan, SNaPshot, microsatellites
Illumina GoldenGate and Infinium
Illumina gene expression analysis
Real time PCR (qPCR)
Mouse genotyping and linkage analysis
> 2.5 billion genotypes since January 1, 2006

11. Microarray Affymetrix Human SNP Array 6.0 now available!
Affymetrix expression and exon arrays
SNP GeneChips and MegAllele
Agilent oligo arrays
Affymetrix Human SNP Array 6.0 now available!
More information and pricing is available on our website.
4,800 arrays processed since January 1, 2006

12. Statistical analysis 12,264 hours of project support
study design consultation
power analysis
analysis of genetic, microarray, genomic copy number, clinical, and proteomic data
12,264 hours of project support
since January 1, 2006

13. Cytogenomics 707 FISH, 1,769 probes labeled since January 1, 2006
karyotyping
FISH and sequential G-to-FISH mapping
fluorescent probe labeling
spectral karyotping (SKY)
breakpoint mapping
array-CGH
707 FISH, 1,769 probes labeled
since January 1, 2006

14. Enabling High Impact Research

15. 970 facility users worldwide (28 countries)
Argentina
Australia
Belgium
Brazil
Canada
China
Costa Rica
Czech Republic
England
Finland
France
Germany
Hong Kong
India
Israel
Italy
Japan
The Netherlands
Peru
Scotland
Singapore
Spain
Sweden
Switzerland
Taiwan
Turkey
USA

16. 22 Genome Canada projects supported

17. The Friendly Faces of TCAG!

18. SickKids Foundation
CIHR
Canadian Genetic
Diseases Network
Genome Canada / OGI
McLaughlin Centre for
Molecular Medicine
CFI / Ontario