1. Volume 9, Issue 5, Pages 649-655 (November 1998)
On the Role of the Pre–T Cell Receptor in αβ versus γδ T Lineage Commitment 
Iannis Aifantis, Orly Azogui, Jacqueline Feinberg, Claude Saint-Ruf, Jan Buer, Harald von Boehmer 
Immunity 
Volume 9, Issue 5, Pages (November 1998)
DOI: /S (00)

2. Figure 1
Flow Cytometric Analysis of Thymocytes from pTα+ and pTα−/− Mice
Total thymocytes from pTα+ and pTα−/− mice were surface-stained with FITC-conjugated anti-TCRγδ antibodies. Cells from pTα+ mice were enriched for the CD4−8− subset by negative depletion of CD4/CD8 positive cells using Dynabeads (Dynal) prior to staining. TCRγδ+ positive cells were sorted according to the indicated gates using a FACS Vantage (Becton Dickinson) cell sorter.
Immunity 1998 9, DOI: ( /S (00) )

3. Figure 2
Assessment of TCRβ Protein Expression in γδ T Cells by Cytoplasmic Staining in pTα+ and pTα−/− Mice
(A) Thymus. (B) Lymph node. Cells were obtained from 6- to 8-week-old pTα−/− mice and wild-type littermate controls. Cells were surface stained with FITC-conjugated anti-TCRγδ; cytoplasmic staining was performed with biotinylated anti-panTCRβ, followed by streptavidin-PE. Percentages were determined by FACScan as indicated. WT, wild-type mice; IC, intracellular.
Immunity 1998 9, DOI: ( /S (00) )

4. Figure 3
Flow Cytometric Analysis of pTα Protein Expression in CD25+ T Cell Progenitors but Not in γδ Thymocytes
(A) Detection of pre-TCRα chain expression by a new MAb on the surface of the TCRβ transfected SCID thymocyte cell line SCB29 (upper panel). Intracellular expression of pre-TCRα protein and TCRβ protein (anti-panTCRβ) in total thymocytes from RAG−/− and pTα−/− mice (lower panel).
(B) Cytoplasmic staining for pre-TCRα protein in γδ thymocytes and CD25+ thymocytes. Cells were stained with the monoclonal antibody against the pre-TCRα chain or with an IgG1κ MAb control with irrelevant specificity (as an indicator of background fluorescence). IC, intracellular.
Immunity 1998 9, DOI: ( /S (00) )

5. Figure 4
DNA Content and Its Correlation with Cytoplasmic Expression of TCRβ Proteins in γδ Cells from pTα+ and pTα−/− Mice
Sorted γδ+ thymocytes from pTα+ and pTα−/− mice were stained with PI and nuclei analyzed for DNA content as described in the Experimental Procedures. Percentages of cells in S+G2/M phases of the cell cycle were determined by FACScan using a doublet discrimination unit.
Immunity 1998 9, DOI: ( /S (00) )