1. GENESDEV/2009/125252_Wu_ Supplemental Fig1
1.5 1
Heme Level * *
0.5
SA (mM):

2. GENESDEV/2009/125252_Wu_ Supplemental Fig2
SiRev-erb
Rev-erb
GSK3

3. GENESDEV/2009/125252_Wu_ Supplemental Fig3
PCDNA3.1
Rev-eb * 2
Fold Repression 1
Reporter: hPGC1-luc
Rev-erb: B 2
PCDNA3.1
Rev-eb *
Fold Repression 1
Rev-erb
hPGC1-luc: WT RREpm RREdm RREp/dm

4. GENESDEV/2009/125252_Wu_ Supplemental Fig4
2.0
SiControl
SiRev-eb
1.5
1.0
ChIP enrichment
0.5
Ab: HDAC Hsp90

5. GENESDEV/2009/125252_Wu_ Supplemental Fig5
shRNA
HDAC3
b-actin B
siRNA
NCoR
b-actin

6. GENESDEV/2009/125252_Wu_ Supplemental Fig63 * 2
PGC1 mRNA 1
Succinylacetone(mM): B 5
mRNA level
DMSO 4
SA+DMSO
SA+Heme 3 * * 2 1
PGC1 Bmal1 C
Dex 1uM ETOH
Heme
PGC1a
Rev-erba
b-actin

7. Oxygen Consumption Rate
GENESDEV/2009/125252_Wu_ Supplemental Fig7
Control-3T3 Cells
1.5
1.0
Oxygen Consumption Rate
Respiration-driven
0.5
DMSO Hemin (2.5M) 7

8. GENESDEV/2009/125252_Wu_ Supplemental Fig8
1.5
Rev-erb mRNA level
1.0
0.5
Day Day Day5

9. * * * * GENESDEV/2009/125252_Wu_ Supplemental Fig9 A B S (%)10 8
S (%) * 6 * 4 2
Control-3T3 REV-3T31 REV-3T32 B
100 80 * * 60
G1(%) 40 20
Control-3T3 REV-3T31 REV-3T32