1. 1. Shen et al. 2006 (MOL Biol Rep (2006) Ginko biloba) 2. Melon
ER retention
Membrane domain
Linker domain
Catalytic domain
HMG-CoA binding
HMG-CoA binding
B Phylogenetic tree of HMGRs from various plants (B) including Eleutherococcus senticosus (AFM77981), Eucommia ulmoides (AAV54051), Antirrhinum majus (ABV25901), Salvia miltiorrhiza (ACD37361), Picrorhiza kurrooa (ABC74565), Panax quinquefolius (ACV65036), Arabidopsis thaliana (AtHMGR1S, At1g76490; AtHMGR2, At2g17370), Morus alba (AAD03789), Litchi chinensis (ABF56518), Hevea brasiliensis (AAU08214), Ricinus communis (EEF52919), fungi Pichia jadinii (O74164), and animals such as Homo sapiens (NP_000850), Drosophila melanogaster (P14773) and Mus musculus (NP_032281). Neighbor-joining method was used and the branch lengths are proportional to divergence, with the scale of 0.1 representing 10% changes.
1. Shen et al (MOL Biol Rep (2006) Ginko biloba)
2. Melon
3. Glycosylation site : Denbow et al. 1996
N-linked-glycosylation site present in the short length of HMGR that is exposed to the lumen of the ER is associated with the production of secondary metabolites, whereas it is absent in the isoforms of HMGR associated with either rapidly growing tissues or the constitutive synthesis of sterols (Denbow et al., 1996).
4. Dale etal. (1995)Leivar et al (2005) H1CD
HMGR1CD domain cloning ESLD
Yujin : PPVMS
NADP(H) binding
NADP(H) binding
NADP(H) binding
Supplemental Figure S5. Multiple alignment of the deduced amino acid sequences of PgHMGR1 and PgHMGR2 with homologous HMGRs from other plants; Eleutherococcus senticosus (EsHMGR, AFM77981), Eucommia ulmoides (EuHMGR, AAV54051), Salvia miltiorrhiza (SmHMGR, ACD37361), Panax quinquefolius (PqHMGR, ACV65036), Camptotheca acuminata (CaHMGR, AAB69727), Solanum chacoense (ScHMGR, AEX26934), and Arabidopsis thaliana (AtHMGR1S, At1g76490; AtHMGR2, At2g17370). Black boxes indicate identical residues; gray boxes indicate identical residues for at least two of the sequences. Conserved domains are indicated with arrowed lines and three conserved motifs are highlighted in colored boxes. Two putative HMGR-CoA-binding sites and two NADP(H)-binding sites are indicated with a thin square box, and the motif responsible for ER retention is highlighted with bold square box. The indicated triangle represents the starting site for the H1CD cloning in this study.