1. Denise Cabeza Nikki Parks Dr. Brent Polk, Advisor
The Effect of Epidermal Growth Factor on Intestinal Epithelial Cell Receptors
Denise Cabeza
Nikki Parks
Dr. Brent Polk, Advisor

2. Overview
Goal-Design an assay to test the redistribution of Epidermal Growth Factor (EGF) receptors in a living cell migrating towards EGF.
What is EGF?

3. Problem
EGF plays key role in Inflammatory Bowel Disease such as Crohne’s Disease, Ulcerative Colitis, and Peptic Ulcers
1-2 million people in U.S. suffer from IBD

4. Demands vs. Wishes Demands Wishes obtain single cell suspension
grow cells on coverslip
observe cell migration through video microscopy
observe redistribution of EGF receptors
Wishes
have substantial amount of cells to be observed
obtain clear images of cell migration/receptor distribution
develop step-by-step procedure for Dunn Chamber experiment

5. Dunn Chamber
Using the Dunn chamber to study the localization of EGF receptors using antibody detection in fixed cells

6. Completed Tasks Performed cell culture on YAMC cells
Performed multiple experiments with Dunn Direct Viewing Chamber
Used video microscope to attempt to view cell migration in Dunn Chamber
Narrowed scope of project

7. Our Problems
Getting cells to plate in ring on coverslip of Dunn Chamber
don’t stick well to glass
don’t grow well in ring

8. Current Work Altering cell plating portion of experiment
Correspondence with Bryce Davis
Successful images of cell migration in Dunn Chamber

9. Future Plans
Using antibody detection to observe redistribution of EGF receptors
Documentation of Dunn Chamber experimental procedure