1. Quantitative Measurement of Cell-Free Plasma DNA and Applications for Detecting Tumor Genetic Variation and Promoter Methylation in a Clinical Setting 
Sunil K. Kadam, Mark Farmen, John T. Brandt 
The Journal of Molecular Diagnostics 
Volume 14, Issue 4, Pages (July 2012)
DOI: /j.jmoldx
Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
Effect of sample collection tube type (EDTA or Streck), DNA extraction method (Nucleospin extraction column, A; and Qiagen DNA extraction kit, B), and storage time on cfDNA measurement. The box plots illustrate the 25th and 75th percentiles, the line within each box is the median value, and the whiskers extend to the outermost data points that fall within the computed distances.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
Effect of centrifugation on cellular depletion and plasma cfDNA extraction for off-site quantification from batched clinical samples. Group A contains samples in which plasma was separated with two centrifugation steps of 1600 × g at the collection site, shipped frozen, and spun at 16,000 × g just before supernatant DNA quantification. Group B contains duplicate samples in which plasma was separated by one centrifugation step at 1600 × g followed by a 16,000 × g spin at the collection site, shipped frozen, and thawed before DNA quantification.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4. Figure 3
A: SpectruMedix electropherograms showing the Surveyor Nuclease digestion products. B: Bidirectional sequence analysis of a known mutation (c.658TAT, p.Y220C) spiked into normal plasma at 100- and 50-ng quantities.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

5. Figure 4
A: SpectruMedix electropherograms showing the Surveyor Nuclease digestion products at mutant (c.658TAT>TGT, p.Y220C) to WT (c658TAT) ratios of 45%, 22.5%, and 11.2%. B: Bidirectional sequence analysis of a known mutation (p.Y220C) spiked into normal plasma at mutant to WT ratios of 45% and 22.5% and the forward primer for 11.2%.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

6. Figure 5
Immunohistochemical detection of p53 protein and molecular characterization of TP53 gene variants in tissue and cfDNA extracted from corresponding samples. A and B: The presence of nuclear p53 in tumor biopsy sections. C and D: DNA sequence trace in the region of exon 5 and exon 8, respectively, from tumor tissue. E and F: cfDNA sequence trace in the region of exon 5 and exon 8, respectively, from corresponding plasma. G and H: DNA sequence trace in the region of exon 5 and exon 8, respectively, from WT reference.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions