1. Characterization of adenosine receptors in bovine chondrocytes and fibroblast-like synoviocytes exposed to low frequency low energy pulsed electromagnetic fields 
K. Varani, Ph.D., M. De Mattei, Ph.D., F. Vincenzi, Sc.D., S. Gessi, Ph.D., S. Merighi, Ph.D., A. Pellati, Sc.D., A. Ongaro, Ph.D., A. Caruso, Ph.D., R. Cadossi, Ph.D., P.A. Borea, Ph.D. 
Osteoarthritis and Cartilage 
Volume 16, Issue 3, Pages (March 2008)
DOI: /j.joca
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

2. Fig. 1
Immunohistochemistry for aggrecan (A), type II (B) and type I (C) collagens of bovine chondrocytes cultured in monolayers. Nuclei were counterstained by hematoxylin.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

3. Fig. 2
Expression of vimentin in synovial fibroblasts (A) (original magnification 20×). Analysis of CD14 mRNA expression (B upper panel) in macrophages (MC) and in bovine fibroblast-like synoviocytes (FS). M is 100bp DNA ladder (Biolabs). One microgram of total RNA was loaded for lane and stained with ethidium bromide to confirm equal RNA quantity used for RT-PCR (B lower panel).
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

4. Fig. 3
Western blotting analysis of bovine chondrocytes and fibroblast-like synoviocytes in the absence and in the presence of PEMFs for A1, A2A and A2B adenosine receptors (A). Each band was quantified by using molecular analyst/PC densitometry software (Bio-Rad). Mean densitometry data from three independent experiments were normalized to control from chondrocytes (B) and fibroblast-like synoviocytes (C).
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

5. Fig. 4
Saturation of [3H]-ZM binding to A2A adenosine receptors (A) and of [3H]-MRE 3008F20 binding to A3 adenosine receptors (C) on untreated or PEMF-treated bovine chondrocyte membranes. Experiments were performed as described in Materials and methods. Values are the means and vertical lines s.e.m. of six separate experiments performed in triplicate. The Scatchard plots of the same data are shown in panels B and D.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

6. Fig. 5
Saturation of [3H]-ZM binding to A2A adenosine receptors (A,B) and of [3H]-MRE 3008F20 binding to A3 adenosine receptors (C,D) on untreated or PEMF-treated bovine fibroblast-like synoviocyte membranes. Experiments were performed as described in Materials and methods. Values are the means and vertical lines s.e.m. of six separate experiments performed in triplicate. The Scatchard plots of the same data are shown in panels B and D.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

7. Fig. 6
Van't Hoff plot showing the effect of temperature on the equilibrium binding association constant, KA=1/KD of [3H]-ZM to A2A adenosine receptors (A,C) or [3H]-MRE 3008F20 to A3 adenosine receptors (B,D) in untreated or PEMF-treated bovine fibroblast-like synoviocyte membranes, respectively. The plots are essentially linear in the temperature range investigated (4–30°C). Binding experiments were performed as described in Materials and methods (n=3).
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

8. Fig. 7
Competition experiments of specific [3H]-ZM to A2A adenosine receptors (A,C) or [3H]-MRE 3008F20 binding to A3 adenosine receptors (B,D) of CGS or Cl-IB-MECA in untreated and PEMF-treated bovine chondrocytes (A,B) or fibroblast-like synoviocyte (C,D) membranes. Competition experiments were performed as described in Materials and methods (n=4).
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

9. Fig. 8
cAMP experiments by using a typical A2A agonist, CGS (A,C) or a typical A3 agonist, Cl-IB-MECA (B,D) in untreated and PEMF-treated bovine chondrocytes (A,B) or fibroblast-like synoviocytes (C,D). cAMP experiments were performed as described in Materials and methods (n=4).
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

10. Fig. 9
Proliferation activity measured by [3H] thymidine incorporation assay. Bovine chondrocytes (A) or fibroblast-like synoviocytes (B) were treated with CGS and Cl-IB-MECA at 10μM concentration.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions