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Polydeoxyribonucleotide restores blood flow in an experimental model of ischemic skin flaps  Francesca Polito, PhD, Alessandra Bitto, MD, PhD, Mariarosaria.

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Presentation on theme: "Polydeoxyribonucleotide restores blood flow in an experimental model of ischemic skin flaps  Francesca Polito, PhD, Alessandra Bitto, MD, PhD, Mariarosaria."— Presentation transcript:

1 Polydeoxyribonucleotide restores blood flow in an experimental model of ischemic skin flaps 
Francesca Polito, PhD, Alessandra Bitto, MD, PhD, Mariarosaria Galeano, MD, Natasha Irrera, JrD, Herbert Marini, MD, Margherita Calò, PhD, Francesco Squadrito, MD, Domenica Altavilla, PhD  Journal of Vascular Surgery  Volume 55, Issue 2, Pages (February 2012) DOI: /j.jvs Copyright © 2012 Society for Vascular Surgery Terms and Conditions

2 Fig 1 A, In this schematic representation of the H-shaped double-flap model, the rectangle represents the ischemic test wound and the area investigated by laser Doppler, and the square inside the rectangle represents the area of region of interest calculation. Laser Doppler images are shown for (B) intact skin and in ischemic flaps at (C) 10 minutes, in ischemic flaps + vehicle at (D) 3, (E) 5, and (F) 10 days after wounding, and in ischemic flaps + polydeoxyribonucleotide at (G) 3, (H) 5, and (I) 10 days after wounding. Low or no perfusion is displayed as dark blue, whereas the highest perfusion interval is displayed as red. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

3 Fig 2 A, Quantitative analysis of blood flow evaluated by laser Doppler is shown at 10 minutes, and at 3, 5, and 10 days after surgical procedures in the various groups. Rats were administered vehicle or polydeoxyribonucleotide (PDRN) starting immediately after surgery. The blood flow of the ischemic flap is expressed as a relative amount compared with the intact skin. *P < .001 vs ischemic flap + vehicle, #P < .01 vs ischemic flap + vehicle. B, Percentage of blood flow vs intact skin evaluated by laser Doppler at 3, 5, and 10 days after surgical procedures. Rats were administered vehicle or PDRN starting immediately after surgery. The blood flow of ischemic flaps is expressed as a relative amount compared with the intact skin. *P < .001 vs ischemic flap + vehicle. The error bars show the standard deviation. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

4 Fig 3 A, Expression of vascular endothelial growth factor (VEGF) messenger RNA is shown at 3, 5, and 10 days after surgery. Bars represent the mean ± standard deviation (error bars) of seven animals. *P < .01 vs ischemic flap + vehicle. B, Western blots show VEGF in ischemic flaps at 3, 5, and 10 days after surgery. Upper panel, Representative autoradiography highlights VEGF expression. Lower panel, Quantitative data represent the mean ± standard deviation (error bars) of seven animals. *P < .05 vs ischemic flap + vehicle. C, Western blots show hypoxia inducible factor-1 (HIF-1α) at 3, 5, and 10 days after surgery. Upper panel, Representative autoradiography highlights HIF-1α expression. Lower panel, Quantitative data represent the mean ± standard deviation (error bars) of seven animals. *P < .05 vs ischemic flap + vehicle. PDRN, Polydeoxyribonucleotide. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

5 Fig 4 A, Western blots show inducible nitric oxide synthase (iNOS) at 3, 5, and 10 days after surgery. Upper panel, Representative autoradiography highlights iNOS expression. Lower panel, Quantitative data represent the mean ± standard deviation (error bars) of seven animals. *P < .01 vs ischemic flap + vehicle. B, Quantitative data of NO products in ischemic flaps are shown at 3, 5, and 10 days after surgery. Data represent the mean ± standard deviation (error bars) of seven animals. #P < .001 vs ischemic flap + vehicle. PDRN, Polydeoxyribonucleotide. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

6 Fig 5 Histologic evaluation (hematoxylin and eosin) of skin wound obtained from ischemic flaps is shown at day 5 and 10 after surgery (original magnification, ×10). A, Ischemic flap + vehicle at day 5 shows the absence of a healing process and a marked inflammatory infiltrate. B, Ischemic flap + polydeoxyribonucleotide (PDRN) at day 5 shows a still-incomplete healing process. C, Ischemic flap + vehicle at day 10 shows a poor healing process, with a sustained inflammatory infiltrate and a marked granulation tissue. D, Ischemic flap + PDRN at day 10 shows a complete healing and a normal skin architecture. Histologic score of (E) granulation tissue thickness and (F) epidermal regeneration were evaluated at day 10 according to the Table criteria. Each bar represents the mean ± standard deviation (error bar) of seven animals *P < .001 vs ischemic flap + vehicle. G, CD31 staining of ischemic flap + vehicle at day 10 shows slight staining of vascular structures. H, Ischemic flap + PDRN at day 10 shows a marked staining of small vessels. I, Microvascular density shows the number of positive capillaries per microscopic field. Each bar represents the mean ± standard deviation (error bars) of seven animals. *P < .05 vs ischemic flap + vehicle. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

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