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Quantitative Fluorescence in Situ Hybridization in Lung Cancer as a Diagnostic Marker
Khuong Truong, Michèle Gerbault-Seureau, Marie-Noëlle Guilly, Philippe Vielh, Gérard Zalcman, Alain Livartowski, Alain Chapelier, Marie-France Poupon, Bernard Dutrillaux, Bernard Malfoy The Journal of Molecular Diagnostics Volume 1, Issue 1, Pages (November 1999) DOI: /S (10)60606-X Copyright © 1999 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions
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Figure 1 Metaphases from lung cancer samples. Images were taken with double filter (FITC-Rhodamine) and a 100× objective. A: Metaphase obtained from an SCLC (case SCLC10).B: Metaphase obtained from NSCLC (case NSCLC1).C: Metaphase obtained from an effusion (EFF1). D:Metaphase obtained from SCLC108 showing an isochromosome i(3q). The Journal of Molecular Diagnostics 1999 1, 33-37DOI: ( /S (10)60606-X) Copyright © 1999 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions
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Figure 2 Scatter plot of the distribution of integrated fluorescence for the long arm (3q) and short arm (3p) paintings, quantified on approximately 500 nuclei of one single population and one pleural effusion containing three subpopulations. Integrated fluorescence is given in arbitrary units (a.u.) on both axes (x axis = 3q and y axis = 3p). A:Case SCLC 6 with Ii = 1.7. B: Same case, Region 1 (normal cells) Ii = 1, region 2 (tumor cells) Ii = 1.5, and region 3 (artifacts) Ii = 0.8. The Journal of Molecular Diagnostics 1999 1, 33-37DOI: ( /S (10)60606-X) Copyright © 1999 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions
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