1. Gene transfer to coronary artery bypass conduits
Cynthia K Chiu-Pinheiro, MD, Timothy O’Brien, MD, PhD, Zvonimir S Katusic, MD, PhD, Luis F Bonilla, MD, Chad E Hamner, MD, Hartzell V Schaff, MD 
The Annals of Thoracic Surgery 
Volume 74, Issue 4, Pages (October 2002)
DOI: /S (02)

2. Fig 1
Saphenous vein grafts harvested 3 days after gene transfer of Ad.CMVLacZ. (A) Adventitial and (B) endothelial transgene expression at 2.5 × 109 pfu/mL. Dose-dependent increase in (C) adventitial and (D) endothelial expression at 5 × 109 pfu/mL. Expression appeared to be greater on the luminal surface. (X-gal stain; magnification, ×10.)
The Annals of Thoracic Surgery  , DOI: ( /S (02) )

3. Fig 2
Microscopic evaluation of saphenous vein graft with eosin counterstaining. (A) No staining for β-galactosidase in a control specimen. In contrast, histologic staining demonstrates dose-dependent localization of β-galactosidase to luminal endothelial cells 3 days after Ad.CMVLacZ transduction at (B) 2.5 × 109 pfu/mL and at (C) 5 × 109 pfu/mL. (Magnification, ×40.)
The Annals of Thoracic Surgery  , DOI: ( /S (02) )

4. Fig 3
β-Galactosidase (B-gal) protein levels in canine saphenous vein coronary artery bypass grafts transduced with the lacZ gene. Saphenous vein grafts were exposed to phosphate-buffered saline with albumin (PBSA) as a control or to Ad.CMVLacZ at 2.5 × 109 pfu/mL or 5 × 109 pfu/mL and were measured for β-galactosidase protein 3 days postoperatively. ∗Significantly different from each other and from phosphate-buffered saline with albumin controls (p < 0.05).
The Annals of Thoracic Surgery  , DOI: ( /S (02) )