1. Volume 60, Issue 4, Pages 856-861 (October 2011)
Methods to Reduce the Contraction of Tissue-Engineered Buccal Mucosa for Use in Substitution Urethroplasty 
Jacob M. Patterson, Anthony J. Bullock, Sheila MacNeil, Christopher R. Chapple 
European Urology 
Volume 60, Issue 4, Pages (October 2011)
DOI: /j.eururo
Copyright © 2011 European Association of Urology Terms and Conditions

2. Fig. 1
The appearance of the experimental-size tissue-engineered buccal mucosa (TEBM) showing significant contraction over 14 d when de-epidermised dermis (DED) was co-cultured with oral keratinocytes and stromal fibroblasts. Control DED (A) did not contract over 14 d (C), but DED plus cells contracted significantly (B, D). (E) A larger TEBM graft of 6×4cm was restrained during culture by suturing it to a stainless steel grid. (F, G) The MTT assay was used to indicate the presence of viable cells on DED: (F) unseeded DED; (G) DED after co-culture with oral keratinocytes and fibroblasts for 14 d. Uniform staining indicates cellular metabolic activity throughout the TEBM.
European Urology  , DOI: ( /j.eururo )
Copyright © 2011 European Association of Urology Terms and Conditions

3. Fig. 2
Cell viability assessed using MTT eluted stain assay. Oral mucosa keratinocytes (E) and fibroblasts (F) were cultured for 48h with human de-epidermised dermis (DED), β-aminopropionitrile (β-APN; 200μg/ml), β-APN plus DED, or DED pretreated with 0.05% or 0.1% glutaraldehyde. Results shown are mean plus or minus standard error of the mean for six replicates.
European Urology  , DOI: ( /j.eururo )
Copyright © 2011 European Association of Urology Terms and Conditions

4. Fig. 3
The time course of tissue-engineered buccal mucosa (TEBM) contraction (A) and the extent of contraction at 28 d compared with the original surface area (B): (A) mean values for each of the treatments versus time; (B) mean plus or minus standard error of the mean for all experiments.
European Urology  , DOI: ( /j.eururo )
Copyright © 2011 European Association of Urology Terms and Conditions

5. Fig. 4
Histologic appearance of tissue-engineered buccal mucosa grafts comparing control (A) with those treated with β-aminopropionitrile (B) or glutaraldehyde at 0.05% (C) and 0.1% (D).
European Urology  , DOI: ( /j.eururo )
Copyright © 2011 European Association of Urology Terms and Conditions