1. Alterations in mouse embryo intracellular pH by DMO during culture impair implantation and fetal growth 
Deirdre L. Zander-Fox, Megan Mitchell, Jeremy G. Thompson, Michelle Lane 
Reproductive BioMedicine Online 
Volume 21, Issue 2, Pages (August 2010)
DOI: /j.rbmo
Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

2. Figure 1
Schematic diagram of experimental design. Arrows indicate control conditions; dotted lines with circles indicate the presence of 2mmol/l 5,5-dimethyl-2,4-oxazolidinedione (DMO).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

3. Figure 2
Effect of continual incubation with 2mmol/l 5,5-dimethyl-2,4-oxazolidinedione (DMO) on intracellular pH at varying stages of development. 2-cell n=22; 8-cell n=14; morula n=16. Values are mean±SEM. ∗Significantly different from control (P<0.05).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

4. Figure 3
Percentage of cells exhibiting apoptosis in blastocysts after varying stages of DMO exposure. Control n=23; zygote to 2-cell n=29; 2-cell to 8-cell n=33; 8-cell to blastocyst n=25; Zygote to blastocyst n=28. Values are mean±SEM (three replicates). ∗Significantly different from control (P<0.05).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

5. Figure 4
The effect of DMO exposure on blastocyst gene expression. Values are mean±SEM based on four replicates with 30 pooled blastocysts per replicate. ∗Significant difference from control (P<0.05).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions