1. IgG Detection Using Anti-IgG Conjugated Microparticles
By Sarah Leung and
Anbar Najam

2. Methods Conjugated anti-IgG antibodies to 0.92 um microparticles
33% microparticle surface coverage with IgG
0.02% microparticle concentration in solution

3. Methods
Positioned a 180 degree optical reflection probe directly above samples for detection on a two-well slide

4. Results
5*10-11 g/mL to 5*10-6 g/mL IgG serial dilutions

5. Results
5*10-15 g/mL to 5*10-6 g/mL IgG serial dilutions

6. Discussion
Results lacked evidence of any trends between antigen concentration and light scattering intensity
Possible explanations:
Coagulation of microparticles
High scattering due to 180 degree detection
Conjugation of antibody, rather than antigen, to microparticles

7. Next Experiment
Added TWEEN 80 to existing 0.92 um microparticle solution and evaluate 180 degree vs. 45 degree light scattering detection
Vs.
180 degrees
45 degrees

8. Results
5*10-14 g/mL to 5*10-6 g/mL IgG serial dilutions

9. Results
5*10-14 g/mL to 5*10-6 g/mL IgG serial dilutions

10. Discussion
180 degree results lacked evidence of any trends between antigen concentration and light scattering intensity
With 45 degree probe, we found a detection limit of g/ml

11. Contact Angle Measurements of Anti-E. Coli and Anti-E
Contact Angle Measurements of Anti-E. Coli and Anti-E. Coli Conjugated Microparticles on Superhydrophobic Surface

12. Methods Conjugated anti-E. coli antibodies to 0.92 um microparticles
33% microparticle surface coverage with anti-E. coli
0.02% microparticle concentration in solution

13. Methods Dispensed 10 ul droplet on to superhydrophobic surface.
Measured contact angle over 10 minutes
Droplets:
Deionized Water
Anti-E. coli -Conc. 5*10-7
Anti-E. coli conjugated to microparticles

14. Results
Anti-E. coli -Conc. 5*10-7

15. Discussion Results oscillate and do not form a smooth curve.
Possible explanations:
Surface contamination
Droplet movement, vibration.
Position of droplet.
Size of droplet

16. Next Experiment
Decrease the volume of the droplet from 10 ul to 3 ul for a more spherical shape.

17. Methods

18. Results
Deionized Water

19. Future Work Minimize the oscillations.
Change the position of the surface.
Determine an efficient way to dispense 3ul droplet on to superhydrophobic surface

20. Acknowledgement s UA/NASA Space Grant Dr. Jeong-Yeol Yoon
Lab group: Tremaine B. Powell, Jin-Hee Han, Brian C. Heinze, Phat L. Tran, Jennine N. Chesler, Dr. Keesung Kim, and Dr. Song