Presentation is loading. Please wait.

Presentation is loading. Please wait.

Controlled diesel exhaust and allergen coexposure modulates microRNA and gene expression in humans: Effects on inflammatory lung markers  Christopher.

Published byMelinda Greer Modified over 6 years ago

Similar presentations

Presentation on theme: "Controlled diesel exhaust and allergen coexposure modulates microRNA and gene expression in humans: Effects on inflammatory lung markers  Christopher."— Presentation transcript:

1 Controlled diesel exhaust and allergen coexposure modulates microRNA and gene expression in humans: Effects on inflammatory lung markers  Christopher F. Rider, PhD, Masatsugu Yamamoto, MD, Oliver P. Günther, PhD, Jeremy A. Hirota, PhD, Amrit Singh, BS, Scott J. Tebbutt, PhD, Chris Carlsten, MD, MPH  Journal of Allergy and Clinical Immunology  Volume 138, Issue 6, Pages (December 2016) DOI: /j.jaci Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 A, Outline of study design. ∗Order randomized. B, Details of the testing and data analysis performed. brush, Epithelial cell brushings; LLL, left lower lobe; RLL, right lower lobe; RML, right middle lobe. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 miRNA and gene expression were measured with NanoString nCounter Human miRNA v2 (A) and Human Immunology v1 (B) assays, respectively, in total RNA extracted from bronchial brushings. Effects of the indicated conditions were evaluated using robust linear models and Venn diagrams constructed showing miRNAs and mRNAs with q values of ≤0.05. Stars indicate genes whose expression was increased and decreased by different conditions. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Mixed models were used to evaluate the effects of allergen or DE exposure on brushing miRNA (A) and mRNA (B) expression. The 20 miRNAs and mRNAs with the smallest uncorrected P values and q values for allergen exposure were plotted (top panels). Values for the same miRNAs and mRNAs following DE exposure are plotted in the bottom panels. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Bar (mean) and whisker (SD) plots showing the effects of conditions on the expression of the 5 miRNAs and mRNAs with the smallest uncorrected P values for allergen were generated. Gray circles indicate individual participant values. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Mixed effects models demonstrated the effects of allergen or DE exposure on brushing miRNA (A) and mRNA (B) expression. The 20 miRNAs and mRNAs with the smallest uncorrected P values and q values for DE exposure were plotted (top panels). Values for the same miRNAs and mRNAs following allergen exposure were also plotted (bottom panels). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig 6 The miRTarBase database was consulted for miRNAs targeting CDKN1C and those modulated by allergen treatment grouped, with shades of blue indicating the strength of experimental evidence. qPCR, Quantitative PCR; qRT-PCR, quantitative RT-PCR. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig 7 A, Correlations between the 20 miRNAs and mRNAs with the lowest P values following allergen exposure were analyzed by PLS regression and the first 2 components plotted as a heatmap. Blue depicts negative correlation, whereas red indicates positive correlation. B, Network diagrams were constructed showing elements with a correlation of ≥0.7 between variables. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Fig 8 A, Correlations between the 20 mRNAs and 13 inflammatory mediators with the lowest P values following allergen exposure were analyzed by PLS regression and the first 2 components plotted as a heatmap. Blue depicts negative correlation, whereas red indicates positive correlation. B, Network diagrams were constructed showing elements with a correlation of ≥0.7 between variables. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Controlled diesel exhaust and allergen coexposure modulates microRNA and gene expression in humans: Effects on inflammatory lung markers  Christopher."

Similar presentations

Endoplasmic reticulum stress influences bronchial asthma pathogenesis by modulating nuclear factor κB activation So Ri Kim, MD, PhD, Dong Im Kim, MS, Mi.

Endoplasmic reticulum stress influences bronchial asthma pathogenesis by modulating nuclear factor κB activation So Ri Kim, MD, PhD, Dong Im Kim, MS, Mi.
MicroRNA signature in patients with eosinophilic esophagitis, reversibility with glucocorticoids, and assessment as disease biomarkers  Thomas X. Lu,

MicroRNA signature in patients with eosinophilic esophagitis, reversibility with glucocorticoids, and assessment as disease biomarkers  Thomas X. Lu,
Classification of childhood asthma phenotypes and long-term clinical responses to inhaled anti-inflammatory medications  Judie A. Howrylak, MD, Anne L.

Classification of childhood asthma phenotypes and long-term clinical responses to inhaled anti-inflammatory medications  Judie A. Howrylak, MD, Anne L.
Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O
Circulating microRNAs as biomarkers in patients with allergic rhinitis and asthma  Ronaldo P. Panganiban, BS, Yanli Wang, BS, Judie Howrylak, MD, PhD,

Circulating microRNAs as biomarkers in patients with allergic rhinitis and asthma  Ronaldo P. Panganiban, BS, Yanli Wang, BS, Judie Howrylak, MD, PhD,
Rachel L. Clifford, PhD, Meaghan J. Jones, PhD, Julia L

Rachel L. Clifford, PhD, Meaghan J. Jones, PhD, Julia L
High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE  Elizabeth A. Erwin,

High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE  Elizabeth A. Erwin,
Kyoung-Bok Min, MD, PhD, Jin-Young Min, PhD 

Kyoung-Bok Min, MD, PhD, Jin-Young Min, PhD 
Fatty acids, inflammation, and asthma

Fatty acids, inflammation, and asthma
Asthma inflammatory phenotypes show differential microRNA expression in sputum  Tania Maes, PhD, Francisco Avila Cobos, MSc, Florence Schleich, MD, PhD,

Asthma inflammatory phenotypes show differential microRNA expression in sputum  Tania Maes, PhD, Francisco Avila Cobos, MSc, Florence Schleich, MD, PhD,
Epigenetic mechanisms silence a disintegrin and metalloprotease 33 expression in bronchial epithelial cells  Youwen Yang, PhD, Hans Michael Haitchi, MD,

Epigenetic mechanisms silence a disintegrin and metalloprotease 33 expression in bronchial epithelial cells  Youwen Yang, PhD, Hans Michael Haitchi, MD,
Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 
Classification of childhood asthma phenotypes and long-term clinical responses to inhaled anti-inflammatory medications  Judie A. Howrylak, MD, Anne L.

Classification of childhood asthma phenotypes and long-term clinical responses to inhaled anti-inflammatory medications  Judie A. Howrylak, MD, Anne L.
Intrinsic atopic dermatitis shows similar TH2 and higher TH17 immune activation compared with extrinsic atopic dermatitis  Mayte Suárez-Fariñas, PhD,

Intrinsic atopic dermatitis shows similar TH2 and higher TH17 immune activation compared with extrinsic atopic dermatitis  Mayte Suárez-Fariñas, PhD,
MicroRNA signature in patients with eosinophilic esophagitis, reversibility with glucocorticoids, and assessment as disease biomarkers  Thomas X. Lu,

MicroRNA signature in patients with eosinophilic esophagitis, reversibility with glucocorticoids, and assessment as disease biomarkers  Thomas X. Lu,
Identification of novel gene signatures in patients with atopic dermatitis complicated by eczema herpeticum  Lianghua Bin, MD, PhD, Michael G. Edwards,

Identification of novel gene signatures in patients with atopic dermatitis complicated by eczema herpeticum  Lianghua Bin, MD, PhD, Michael G. Edwards,
FcεRI-mediated amphiregulin production by human mast cells increases mucin gene expression in epithelial cells  Shigeru Okumura, DDS, PhD, Hironori Sagara,

FcεRI-mediated amphiregulin production by human mast cells increases mucin gene expression in epithelial cells  Shigeru Okumura, DDS, PhD, Hironori Sagara,
Dupilumab improves the molecular signature in skin of patients with moderate-to-severe atopic dermatitis  Jennifer D. Hamilton, PhD, Mayte Suárez-Fariñas,

Dupilumab improves the molecular signature in skin of patients with moderate-to-severe atopic dermatitis  Jennifer D. Hamilton, PhD, Mayte Suárez-Fariñas,
MicroRNA activation signature in patients with hemophagocytic lymphohistiocytosis and reversibility with disease-specific therapy  Janos Sumegi, MD, PhD,

MicroRNA activation signature in patients with hemophagocytic lymphohistiocytosis and reversibility with disease-specific therapy  Janos Sumegi, MD, PhD,
Julie Wang, MD, James H. Godbold, PhD, Hugh A. Sampson, MD 

Julie Wang, MD, James H. Godbold, PhD, Hugh A. Sampson, MD 

Similar presentations

Ads by Google