1. ToxoDB
ApiDB Workshop
June 2006

2. Shotgun Sequencing Strategy
genetic markers
C810-A6
SAG4
Bs328
sourceDNA
end sequencing
of small insert
plasmid library
end sequencing
of larger insert libraries
(BAC/cosmid/plasmid)
assembly
scaffold
physical gap
sequence gaps

3. Sequencing Scaffolds Pinned to the Genetic Map:
chromosomes 1a & 1b
(Sanger)
whole genome shotgun
(TIGR)
unmapped markers
(telomeric?)
mapping ambiguities
Sequencing & assembly: Ian Paulsen, Neil Hall
Genetic map: Chunlei Su & David Sibley
Mapping: Bindu Gajria & David Roos

4. Improved Genetic & Physical Maps
A. Khan et al (2005)
Nucl. Acids Res. 33:2980

5. A. Khan et al (2005) Nucl. Acids Res. 33:2980