1. Statistical analysis (PERMANOVA) for the potential of black soldier fly larvae (BSFL) as a shrimp feed ingredient to replace partial fishmeal Bell Lin

2. Goal & HoHa
Using statistic approach PERMANOVA to find the difference of shrimp growth and survival performance between four diets
HO: No statistic difference between four shrimp diet treatments on shrimp performance
HA: There is a pattern between four shrimp diet treatments

3. Dataset Description 16 samples and 4 variables
Experiment duration: 84 days
Four groups of samples, each group has four replicates
CTL: Control group diet
25BSF: 25% of the fish meal was replace by BSF
50BSF: 50% of the fish meal was replace by BSF
75BSF: 75% of the fish meal was replace by BSF
Variables:
Survival: Shrimp survival rate (%)
Yield m2: Shrimp Yield in grams/m2
SGR d: Specific Growth Rate per day (%)
FCR: Feed conversion rate (%)
Main Matrix
Second Matrix

4. Dataset Processing No samples and outliers discarded.
Large Skewness for SGRd and FCR
Tried to perform log transformation for SGRd and FCR columns but didn’t change the skewness much.
Performed general relativization

5. Dataset Analysis

6. Results Interpretation
F(12,3) = 1.806
Pseudo F range from 0 to 100
P-value not significant (larger than 0.05)

7. Results Interpretation

8. Results Interpretation
Not significant between any of the groups!

9. Discussion
Fail to reject Ho: No statistic difference between diet groups on shrimp performances.
What I learned:

10. Next step Re-analysis: Perform ANOVA, look at one variables at a time.
Formulate a feed formulate replacing fish meal with 100% BSFL.
Replace different proportion of fish meal with BSFL on different aquatic species.

11. Q&A