1. 2. Electron Microscopy

2. A. Transmission electron Microscopy (TEM)

3. LM vs. TEM

4. TEM Fixatives:

5. 3-D reconstruction of a mitochondrion from TEM sections

6. Immunogold
localization
(TEM)

7. Replica technique for surfaces

8. TEM of replica of freeze-fractured chloroplast

9. TEM of negatively stained actin filaments
(Uranium acetate)

10. B. Scanning Electron Microscopy

11. SEM
DIC TEM

12. Radioisotope labeling

13. Radioisotopically
labeled
molecules

14. EM
Autoradiography Labeled
Pancreatic beta Insulin:
cells pulsed w
3H-leucine for ’
5 min, followed grains over
by 10’ and 45’ Golgi app.
chase. Sections
coated w photo
emulsion and ’
exposed in dark Grains over
Grains secretory
developed and granuled
photographed
in TEM