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Quantification of Melanoma mRNA Markers in Sentinel Nodes

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Presentation on theme: "Quantification of Melanoma mRNA Markers in Sentinel Nodes"— Presentation transcript:

1 Quantification of Melanoma mRNA Markers in Sentinel Nodes
Helene N. Abrahamsen, Ebba Nexo, Torben Steiniche, Stephen J. Hamilton-Dutoit, Boe S. Sorensen  The Journal of Molecular Diagnostics  Volume 6, Issue 3, Pages (August 2004) DOI: /S (10) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Assay linearity and effect of analysis of melanoma cell (SK-MEL-28) titrations with (in 2 μg lymph node-derived RNA) and without (in water) lymphoid RNA matrix. Observed values for samples with and without lymphoid matrix are based on data from the quantitative real-time RT-PCR. Expected values are calculated from titrations in water (see Material and Methods). Data are presented for two experimental runs, quantifying MART-1 and tyrosinase mRNA. Values are given in arbitrary units (arb.u.) (defined in Materials and Methods) measured in water or in 2 μg lymphoid RNA. Each data point represents the mean of five replicates ± SD. Correlation of fit (r2) was ≥0.99 for all regression lines, except for MART-1with lymphoid matrix (r2 = 0.96). For tyrosinase, regression lines with and without lymphoid matrix were significantly different (P < 0.05), whereas no difference was shown for MART-1 regression lines. The Journal of Molecular Diagnostics 2004 6, DOI: ( /S (10) ) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Quantification of melanocyte-associated differentiation marker mRNAs in sentinel nodes. MART-1 and tyrosinase mRNAs were quantified in frozen tissue from 30 nodes, classified after extensive histological examination of the paraffin-embedded half as follows: 10 with metastatic melanoma, 10 with benign nevus inclusions, and 10 with no histological evidence of intranodal melanocytes. Values are given in arbitrary units (arb.u.) (defined in Materials and Methods) present in 2 μg lymphoid RNA. Vertical lines represent median values. Medians for sentinel nodes without melanocytic cells were zero arb.u. for both MART-1 and tyrosinase. The Journal of Molecular Diagnostics 2004 6, DOI: ( /S (10) ) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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