1. Real-Time Imaging of Dendritic Cell Responses to Sterile Tissue Injury
Chi Ching Goh, Jackson LiangYao Li, Sapna Devi, Nadja Bakocevic, Peter See, Anis Larbi, Wolfgang Weninger, Florent Ginhoux, Veronique Angeli, Lai Guan Ng 
Journal of Investigative Dermatology 
Volume 135, Issue 4, Pages (April 2015)
DOI: /jid
Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Migration of dermal dendritic cells (DDCs) in the dermis after laser-induced sterile injury. Maximum z-projected snapshots from time-lapse sequence showing distribution of DDCs at (a) resting state, (b) 50 min post injury, and (c) 3 h post injury. YFP (yellow, DDCs), second harmonic generation (SHG, blue, collagen), and Evans blue (gray, blood vessels, 1 mg ml−1). Bar = 50 μm. (d) Representative velocity of DDCs at steady state and post injury. (e) Representative meandering index of DDCs at steady state and post injury. A value of 1 indicates a straight-line path, whereas a value of 0 indicates random walk. Horizontal line indicates mean. ****P<0.0001; the Mann–Whitney test. Tracking analysis of DDCs (f) responding to injury with no treatment, (g) after pertussis toxin (PTX) treatment, and (h) with phosphate-buffered saline (PBS) as control. White boxes indicate position of injury. Elapsed time shown in hh:mm format. n=3 for each treatment.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Neutrophil-independent dermal dendritic cells (DDCs) migration toward sterile injury. Maximum z-projected snapshots from time-lapse sequence showing (a–c) recruited DDCs (yellow) and neutrophils (red) post injury (d, e) with superimposed migration routes of neutrophils in green and DDCs in cyan. (f) Recruitment of DDCs to injury in a neutrophil-depleted mouse. SHG (blue, collagen) and Evans blue (gray, blood vessels). Bar = 50 μm. (g) Response time of DDCs, defined as the time point at which the distance traveled by individual DDCs first exceeds 5 μm min−1. (h) Chemotactic index of DDCs toward laser injury. A value of 1 indicates a straight-line path, whereas a value of 0 indicates random walk. (g, h) Data includes DDCs that traveled a minimum distance of 30 μm. Each data point represents the average from an independent imaging experiment. Error bar denotes SEM. (i) Average velocity over time. n=3 per condition.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions