1. Peripheral Blood CD34+ Cell Enumeration as a Predictor of Apheresis Yield: An Analysis of More Than 1,000 Collections 
Peter Gambell, Kirsten Herbert, Michael Dickinson, Kerrie Stokes, Mathias Bressel, Dominic Wall, Simon Harrison, H. Miles Prince 
Biology of Blood and Marrow Transplantation 
Volume 18, Issue 5, Pages (May 2012)
DOI: /j.bbmt
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2. Figure 1
Single-platform CD34 ISHAGE-based gating strategy. Viable cells were analyzed using a gate based on 7AAD exclusion. A gate was then drawn on CD45+ cells (R1) to exclude noncellular debris and unlysed red cells. A gate was then drawn to capture a discrete cluster of CD34+ cells with low side scatter (R2). These cells were further refined using a homogenous cluster based on intermediate CD45+ cell expression (R3), and the CD34+ HPCs (R4) were finally defined using intermediate forward scatter (final CD34+ HPCs compared with lymphoid population follicular stem cells). The number of events in this R4 gate were compared with the number of events counted in the singlet bead gate (R7) to derive the reported CD34+ cell count.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
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3. Figure 2
(A) Non–log-transformed PB CD34+ cell counts versus collection CD34+ cell counts. (B) Non–log-transformed PB CD34+ cell counts versus collection CD34+ cell counts for all samples with PB CD34+ cell count <300 × 106/L. (C) Relationship between collection CD34+ cell count and PB CD34+ cell count for all samples (after logarithmic transformation); y = − x; R2 = 91.9%; P <
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
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4. Figure 3
(A) Relationship between collection CD34+ cell count and PB CD34+ cell count for all samples from patients mobilized using filgrastim alone (after logarithmic transformation); y = − x; R2 = 89.6%; P < (B) Relationship between collection CD34+ cell count and PB CD34+ cell count for all samples from patients mobilized using filgrastim + chemotherapy (after logarithmic transformation); y = − x; R2 = 91.6%; P <
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
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5. Figure 4
(A) Relationship between collection CD34+ cell count and PB CD34+ cell count for all samples from patients weighing <70 kg (after logarithmic transformation; y = − x; R2 = 90.8%; P < .0001). (B) Relationship between collection CD34+ cell count and PB CD34+ cell count for all samples from patients weighing kg (after logarithmic transformation; y = − x; R2 = 92.1%; P < .0001). (C) Relationship between collection CD34+ cell count and PB CD34+ cell count for all samples from patients weighing >100 kg (after logarithmic transformation; y = − x; R2 = 94.9%; P < .0001).
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
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6. Figure 5
(A) Relationship between collection CD34+ cell count and PB CD34+ cell count for all samples from patients with WCC <20 × 109/L (after logarithmic transformation; y = − x; R2 = 92.1%; P < .0001). (B) Relationship between collection CD34+ cell count and PB CD34+ cell count for all samples from patients with WCC × 109/L (after logarithmic transformation; y = − x; R2 = 93.7%; P < .0001). (C) Relationship between collection CD34+ cell count and PB CD34+ cell count for all samples from patients with WCC >50 × 109/L (after logarithmic transformation; y = − x; R2 = 90.4%; P < .0001).
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
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7. Figure 6
(A) Relationship between collection CD34+ cell count and PB CD34+ cell count for all samples from patients with a diagnosis of NHL (after logarithmic transformation; y = − x; R2 = 92.1%; P < .0001). (B) Relationship between collection CD34+ cell count and PB CD34+ cell count for all samples from patients with a diagnosis of plasma cell dyscrasia (after logarithmic transformation), y = − x, R2 = 92.3%, (P < .0001). (C) Relationship between collection CD34+ cells and PB CD34+ cells for all samples from patients with a diagnosis other than plasma cell dyscrasias or NHL (after logarithmic transformation; y = − x; R2 = 90.0%; P < .0001).
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
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8. Figure 7
Boxplots showing median, 10th and 90th percentiles, maximum, and minimum CE for collections in patients with NHL, MM, and MDS/MPD. NHL versus MM: P = not significant; ∗NHL versus MDS-MPD: P = .015; ∗∗MM versus MDS-MPD: P = .019.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
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