1. Treg and Teff cell subsets show increased TCR overlap during colitis.
Treg and Teff cell subsets show increased TCR overlap during colitis. (A to C) Analysis of TCRα repertoires from Tnaïve (CD44lo CD62hi), Treg (Foxp3+), and Teff (CD44hi CD62Llo) cells in the cLP of TCli TCRβ Foxp3IRES-Thy1.1Tcra+/− mice 2 weeks after initiation of IgG or DSS + αIL10R. n = 5 and 6. (A) Morisita-Horn similarity comparison between two different T cell subsets within each mouse (left) or between different mice within each T cell subset (right). An index value of 1 indicates that the two samples are completely similar, and an index value of 0 means that they are completely dissimilar (left: P = 0.009, Student’s t test; n = 5 and 6; right: P = 0.033, P = 0.014, Student’s t test; n = 10 and 15). (B) Heat map showing the top 25 Teff TCRs in one mouse per row and their corresponding percentage in the Treg subset. Each column does not represent one TCR across all mice. (C) Percentage of T7-1 TCR in repertoire of Tnaïve, Treg, and Teff cell subsets. Each dot represents data from an individual mouse. (D) In vivo analysis of T7-1 TCR. T7-1 was retrovirally transduced into in vitro activated naïve TCliβ Rag1−/− Tg cells, and 2 × 105 cells were transferred into IgG or DSS + αIL10R hosts as indicated in Fig. 1A. Seven days after transfer, cells from the cLP were analyzed by flow cytometry for Teff and Treg cells, and the in vivo frequency among the host CD4+ T cells (P = 0.029, Student’s t test; n = 4). Bars indicate mean ± SEM. *P < 0.05, **P < 0.01.
Jiani N. Chai et al. Sci. Immunol. 2017;2:eaal5068
Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works